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Comparison of Various Reducing Agents for Methane Production by Methanothermobacter marburgensis

Biological methanation is driven by anaerobic methanogenic archaea, cultivated in different media, which consist of multiple macro and micro nutrients. In addition, a reducing agent is needed to lower the oxidation–reduction potential (ORP) and enable the growth of oxygen-sensitive organisms. Until...

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Published in:Microorganisms (Basel) 2023-10, Vol.11 (10), p.2533
Main Authors: Mock, Maximilian Peter, Ochi, Rayen, Bieringer, Maria, Bieringer, Tim, Brotsack, Raimund, Leyer, Stephan
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description Biological methanation is driven by anaerobic methanogenic archaea, cultivated in different media, which consist of multiple macro and micro nutrients. In addition, a reducing agent is needed to lower the oxidation–reduction potential (ORP) and enable the growth of oxygen-sensitive organisms. Until now, sodium sulfide (Na2S) has been used mainly for this purpose based on earlier published articles at the beginning of anaerobic microbiology research. In a continuation of earlier investigations, in this study, the usage of alternative reducing agents like sodium dithionite (Na2S2O4) and L-Cysteine-HCl shows that similar results can be obtained with fewer environmental and hazardous impacts. Therefore, a newly developed comparison method was used for the cultivation of Methanothermobacter marburgensis. The median methane evolution rate (MER) for the alternatives was similar compared to Na2S at different concentrations (0.5, 0.25 and 0.1 g/L). However, the use of 0.25 g/L Na2S2O4 or 0.1 g/L L-Cys-HCl led to stable MER values over consecutive batches compared to Na2S. It was also shown that a lower concentration of reducing agent leads to a higher MER. In conclusion, Na2S2O4 or L-Cys-HCl can be used as a non-corrosive and non-toxic reducing agent for ex situ biological methanation. Economically, Na2S2O4 is cheaper, which is particularly interesting for scale-up purposes.
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subjects Alternative energy sources
anaerobic media
Archaea
Biogas
biological methanation
Carbon dioxide
Chemicals
Culture media
Dithionite
Emissions
Experiments
Gases
Hydrogen
L-cysteine-HCl
Methanation
Methane
Methanogenic archaea
Methanothermobacter
Microbiology
Nutrients
Organisms
Oxidation
Reagents
reducing agent
Reducing agents
Sodium
Sodium dithionite
Sodium sulfide
Stainless steel
Toxicity
Trace elements
title Comparison of Various Reducing Agents for Methane Production by Methanothermobacter marburgensis
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