Reproductive Strategy Inferred from Major Histocompatibility Complex-Based Inter-Individual, Sperm-Egg, and Mother-Fetus Recognitions in Giant Pandas ( Ailuropoda melanoleuca )

• Published in: Cells (Basel, Switzerland) Switzerland), 2019-03, Vol.8 (3), p.257
• Main Authors: Zhu, Ying, Wan, Qiu-Hong, Zhang, He-Min, Fang, Sheng-Guo
• Format: Article
• Language: English
• Subjects: Ailuropoda melanoleuca; Alleles; Animal behavior; Animal reproduction; Animals; Antigens; DQA1 protein; Eggs; Female; Females; Fetus - physiology; Fetuses; Genetic Variation; Genomes; giant panda; Haplotypes; Haplotypes - genetics; Heterozygosity; Heterozygote; Histocompatibility antigen HLA; Homozygote; Hypotheses; Immunological tolerance; Inbreeding; long-fragment super haplotype; Major histocompatibility complex; Major Histocompatibility Complex - genetics; Male; Males; Mate selection; Mating; MHC-I- and MHC-II-dependent inter-individual recognition; MHC-I-based mother-fetus recognition; MHC-II-associated sperm-egg recognition; Microsatellite Repeats - genetics; Microsatellites; Ovum - physiology; Pandas; Pathogens; Reproduction - genetics; Reproductive strategy; Sperm; Spermatozoa - physiology; Ursidae - genetics; Ursidae - physiology; Wildlife conservation
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells8030257

Few major histocompatibility complex (MHC)-based mate choice studies include all MHC genes at the inter-individual, sperm-egg, and mother-fetus recognition levels. We tested three hypotheses of female mate choice in a 17-year study of the giant panda ( ) while using ten functional MHC loci (four MHC class I loci: -C, -F, -I, and -L; six MHC class II loci: -DRA, -DRB3, -DQA1, -DQA2, -DQB1, and -DQB2); five super haplotypes (SuHa, SuHaI, SuHaII, DQ, and DR); and, seven microsatellites. We found female choice for heterozygosity at -C, -I, and DQ and for disassortative mate choice at -C, DQ, and DR at the inter-individual recognition level. High mating success occurred in MHC-dissimilar mating pairs. No significant results were found based on any microsatellite parameters, suggesting that MHCs were the mate choice target and there were no signs of inbreeding avoidance. Our results indicate -DQA1- and -DQA2-associated disassortative selection at the sperm-egg recognition level and a possible -C- and -I-associated assortative maternal immune tolerance mechanism. The MHC genes were of differential importance at the different recognition levels, so all of the functional MHC genes should be included when studying MHC-dependent reproductive mechanisms.