Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB

• Published in: Scientific reports 2017-08, Vol.7 (1), p.7547-11, Article 7547
• Main Authors: Zou, Haitao, Su, Ruixue, Ruan, Jing, Shao, Hongxia, Qian, Kun, Ye, Jianqiang, Yao, Yongxiu, Nair, Venugopal, Qin, Aijian
• Format: Article
• Language: English
• Subjects: 13/1; 13/106; 13/109; 13/2; 13/21; 13/31; 13/44; 13/95; 14/63; 631/80/82/23; 631/80/86; Adaptor Proteins, Vesicular Transport - genetics; Adaptor Proteins, Vesicular Transport - metabolism; Animals; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Avian Proteins - genetics; Avian Proteins - metabolism; Caspase; Cell Line, Tumor; Cell proliferation; Chickens; Double-stranded RNA; Humanities and Social Sciences; Immune response; Interferon; Interleukin 1; Lymphocytes T; Lymphoma; Lymphoma, T-Cell - genetics; Lymphoma, T-Cell - metabolism; Lymphoma, T-Cell - pathology; Marek's disease; multidisciplinary; NF-kappa B - genetics; NF-kappa B - metabolism; NF-κB protein; Poly I-C - pharmacology; Polyinosinic:polycytidylic acid; Poultry; RNA, Double-Stranded - genetics; Science; Science (multidisciplinary); Signal Transduction - drug effects; Signal Transduction - genetics; T cell receptors; T-cell lymphoma; TLR3 protein; Toll-Like Receptor 3 - agonists; Toll-Like Receptor 3 - metabolism; Toll-like receptors; Tumor cells
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-07919-w

Toll-like receptor-3 (TLR3), a member of the pathogen recognition receptor family, has been reported to activate immune response and to exhibit pro-apoptotic activity against some tumor cells. However it is unclear whether TLR3 has same function against chicken lymphoma. In this paper we investigated the effect of TLR3 activation on a Marek’s disease lymphoma-derived chicken cell line, MDCC-MSB1. The TLR3 agonist poly (I:C) activated TLR3 pathway and inhibited tumor cells proliferation through caspase-dependent apoptosis. Using pharmacological approaches, we found that an interferon-independent mechanism involving Toll-IL-1-receptor domain-containing adapter-inducing IFN-α (TRIF) and nuclear factor κB (NF-κB) causes the apoptosis of MDCC-MSB1 cells. This is the first report about the function of TLR3 in chicken T-cell lymphoma, especially in signal pathway. The mechanisms underlying TLR3-mediated apoptosis may contribute to the development of new drug to treat lymphomas and oncovirus infections.