Quantitative analysis of exosomes in the aqueous humor of Korean patients with pseudoexfoliation glaucoma

We aimed to quantitatively analyze the exosome and its cargo in individual aqueous humor (AH) samples from pseudoexfoliation (PEX) glaucoma patients compared to controls using a novel detection platform. We investigated the size distribution and measured the quantitative exosome particle counts in e...

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Bibliographic Details
Published in:Scientific reports 2022-07, Vol.12 (1), p.12875-12875, Article 12875
Main Authors: An, Hyo Jung, Cho, Hyun-kyung, Song, Dae Hyun, Kee, Changwon
Format: Article
Language:English
Subjects:
631/1647/350/354
692/699/3161/3172
CD63 antigen
CD9 antigen
Exosomes
Glaucoma
Humanities and Social Sciences
multidisciplinary
Patients
Quantitative analysis
Science
Science (multidisciplinary)
Size distribution
Surgery
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Summary:We aimed to quantitatively analyze the exosome and its cargo in individual aqueous humor (AH) samples from pseudoexfoliation (PEX) glaucoma patients compared to controls using a novel detection platform. We investigated the size distribution and measured the quantitative exosome particle counts in each AH sample. AH (80–120 µL) was obtained during cataract surgery or glaucoma filtering surgery from 12 Korean subjects (six with PEX glaucoma and six age-matched controls). The mean size of the exosomes was 58.9 ± 18.5 nm measured by a tangential flow filtration system using single-particle interferometric reflectance imaging sensor. Exosome particle count in each CD 63, CD 81, and CD9 spot was significantly greater in PEX glaucoma than in controls in total, CD 63, CD9, syntenin, and scattering(all p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-17063-9