Association of cellular HIV-1 DNA and virological success of antiretroviral treatment in HIV-infected sub-Saharan African adults

• Published in: BMC infectious diseases 2022-01, Vol.22 (1), p.100-100, Article 100
• Main Authors: Moh, Desmorys Raoul, Ntakpé, Jean-Baptiste, Gabillard, Delphine, Yayo-Emieme, Arlette Ahoubet, Badjé, Anani, Kouame, Gérard M, d'Aquin, Toni Thomas, Danel, Christine, Anglaret, Xavier, Eholié, Serge P
• Format: Article
• Language: English
• Subjects: Acquired immune deficiency syndrome; Adults; Africa; Africa South of the Sahara; AIDS; Antiretroviral agents; Antiretroviral drugs; Antiretroviral therapy; Antiviral agents; Care and treatment; CD4 antigen; Deoxyribonucleic acid; Diagnosis; DNA; DNA, Viral - genetics; Dosage and administration; Failure; HIV; HIV infection; HIV Infections - drug therapy; HIV patients; HIV-1 - genetics; HIV-1 DNA; Human immunodeficiency virus; Humans; Immunocompromised host; Immunocompromised hosts; Infectious diseases; Leukocytes, Mononuclear; Life Sciences; Morbidity; Multivariate analysis; Patients; Peripheral blood mononuclear cells; Plasma; Ribonucleic acid; Risk factors; RNA; Sensitivity analysis; Sexually transmitted diseases; STD; Success; Test and treat; Therapeutic success
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-022-07082-2

HIV-1 DNA persists in infected cells, forming viral reservoirs. Pre-antiretroviral treatment (ART) HIV-1 DNA load was reported to predict ART success in European severely immunocompromised patients. The aim of this study was to determine whether HIV-1 DNA levels are associated with virological success in less severely immunocompromised patients who receive early ART in sub-Saharan Africa. The association between pre-ART HIV-1 DNA and the virological response after 30 months on ART was studied in multivariate logistic regression in patients randomised to immediate ART groups in the Temprano trial, which assessed the benefits of early ART in HIV-infected adults in Côte d'Ivoire. HIV-1 DNA was quantified in peripheral blood mononuclear cell (PBMC) using real-time PCR. HIV-1 DNA levels were measured in 1013 patients. Their medians [IQR] of pre-ART CD4 count, HIV-1 RNA and HIV-1 DNA levels were 465 [379-578]/mm , 4.7 [4.0-5.3] log copies/ml and 2.9 [2.5-3.2] log copies/million PBMC, respectively. Pre-ART HIV-1 DNA was significantly correlated with pre-ART HIV-1 RNA (R = 0.59, p