Dielectrophoresis-Based Biosensor for Detection of the Cancer Biomarkers CEA and CA 242 in Serum

We show that dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism for detection of the concentration levels of the pancreatic cancer biomarkers cancer antigen (CA) 242 and carcinoembryonic antigen (CEA) in serum. We noticed a frequency dependence of the negative DEP force appl...

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Bibliographic Details
Published in:Chemosensors 2022-03, Vol.10 (3), p.104
Main Authors: Gudagunti, Fleming Dackson, Gundlakunta, Srilakshmi G., Lima, Ivan T.
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Automation
Binding sites
Biomarkers
Biosensors
Cancer
Carcinoembryonic antigen
Clinical medicine
Dielectrophoresis
Electric fields
Electrodes
Frequency dependence
image processing
Immunoassay
Medical prognosis
Microspheres
Pancreatic cancer
pancreatic cancer biomarkers
Polystyrene
Polystyrene resins
Signal processing
Spectroscopy
Tumors
Vortices
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Summary:We show that dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism for detection of the concentration levels of the pancreatic cancer biomarkers cancer antigen (CA) 242 and carcinoembryonic antigen (CEA) in serum. We noticed a frequency dependence of the negative DEP force applied by interdigitated electrodes on functionalized polystyrene microspheres (PM) with respect to changes in the number of these cancer antigens bound to the PM. An electrode array with a well-defined gradient of the electric field was designed and used, which enabled the automation of the signal processing and reproducibility of the signal acquired by the biosensor.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors10030104