YES-10, A Combination of Extracts from Clematis mandshurica RUPR. and Erigeron annuus (L.) PERS., Prevents Ischemic Brain Injury in A Gerbil Model of Transient Forebrain Ischemia

• Published in: Plants (Basel) 2020-01, Vol.9 (2), p.154
• Main Authors: Lee, Tae-Kyeong, Park, Joon Ha, Kim, Bora, Park, Young Eun, Lee, Jae-Chul, Ahn, Ji Hyeon, Park, Cheol Woo, Noh, Yoohun, Lee, Ji-Won, Kim, Sung-Su, Kim, Jong Dai, Won, Moo-Ho
• Format: Article
• Language: English
• Subjects: asteraceae; common carotid artery occlusion; pyramidal neurons; ranunculaceae; reactive gliosis
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants9020154

RUPR. (CMR) and (L.) PERS. (EALP) have pharmacological effects including anti-inflammatory activity and been used in traditional medicines in Asia. However, neuroprotective effects of CMR and/or EALP extracts against brain ischemic insults have never been addressed. Thus, the aim of this study was to examine neuroprotective effects of YES-10, a combination of extracts from CMR and EALP (combination ratio, 1:1), in the hippocampus following ischemia/reperfusion in gerbils. Protection of neurons was investigated by cresyl violet staining, fluoro-jade B histofluorescence staining and immunohistochemistry for neuronal nuclei. In addition, attenuation of gliosis was studied by immunohistochemistry for astrocytic and microglial markers. Treatments with 50 or 100 mg/kg YES-10 failed to protect neurons in the hippocampus after ischemia/reperfusion injury. However, administration of 200 mg/kg YES-10 protected neurons from ischemia/reperfusion injury and attenuated reactive gliosis. These findings strongly suggest that a combination of extracts from CMR and EALP can be used as a prevention approach/drug against brain ischemic damage.