Evidence for a gut microbial community conferring adaptability to diet quality and temperature stressors in phytophagous insects: the melon fruit fly Zeugodacus cucurbitae (Diptera: Tephritidae) as a case study

• Published in: BMC microbiology 2024-12, Vol.24 (1), p.514-15
• Main Authors: Hafsi, Abir, Moquet, Laura, Hendrycks, Wouter, De Meyer, Marc, Virgilio, Massimiliano, Delatte, Hélène
• Format: Article
• Language: English
• Subjects: Adaptability; Adaptation; Adaptation, Physiological; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; Biological diversity; Case studies; Climate change; Composition; Control; Diet; Diseases and pests; Ecology; Environmental aspects; Environmental conditions; Environmental stress; Evolutionary biology; Experiments; Fecundity; Female; Females; Fertility; Fruit-flies; Gastrointestinal Microbiome - drug effects; Genetic aspects; Growth; Humidity; Identification and classification; Insect adaptation; Insect ecology; Insects; Intestinal microflora; Invasiveness; Islands; Laboratories; Longevity; Longevity - drug effects; Low protein diet; Melons; Metabarcoding; Microbial symbiont; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Nutrient deficiency; Physiology; Proteins; RNA; RNA, Ribosomal, 16S - genetics; rRNA; Stress, Physiological; Sucrose; Symbionts; Symbiosis; Temperature; Temperature effects; Tephritidae - microbiology; Tephritidae - physiology; Zeugodacus cucurbitae
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-024-03673-y

The high invasiveness of phytophagous insects is related to their adaptability to various environments, that can be influenced by their associated microbial community. Microbial symbionts are known to play a key role in the biology, ecology, and evolution of phytophagous insects, but their abundance and diversity are suggested to be influenced by environmental stressors. In this work, using 16 S rRNA metabarcoding we aim to verify (1) if laboratory rearing affects microbial symbiont communities of Zeugodacus cucurbitae females, a cosmopolitan pest of cucurbitaceous crops (2) if temperature, diet quality, and antibiotic treatments affect microbial symbiont communities of both laboratory and wild populations, and (3) if changes in microbial symbiont communities due to temperature, diet and antibiotic affect longevity and fecundity of Z. cucurbitae. The results showed that microbial diversity, particularly the β-diversity was significantly affected by insect origin, temperature, diet quality, and antibiotic treatment. The alteration of gut microbial symbionts, specifically Enterobacteriaceae, was associated with low fecundity and longevity of Z. cucurbitae females feeding on optimal diet only. Fecundity reduction in antibiotic treated females was more pronounced when flies were fed on a poor diet without protein. our study proves the relationship between gut microbiome and host fitness under thermal and diet fluctuation highlighting the importance of microbial community in the adaptation of Z. cucurbitae to environmental stress. Not applicable.