The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells

Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able...

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Bibliographic Details
Published in:Nutrients 2023-05, Vol.15 (11), p.2557
Main Authors: Greco, Stefania, Pellegrino, Pamela, Giampieri, Francesca, Capocasa, Franco, Delli Carpini, Giovanni, Battino, Maurizio, Mezzetti, Bruno, Giannubilo, Stefano Raffaele, Ciavattini, Andrea, Ciarmela, Pasquapina
Format: Article
Language:English
Subjects:
3D culture
Analysis
Apoptosis
Cancer therapies
Cells
Cultivars
Deoxyribonucleic acid
DNA
Extracellular matrix
Female
Fibroids
Fluorescence
Fragaria - chemistry
Fruits
Gene expression
Humans
Leiomyosarcoma
Leiomyosarcoma - drug therapy
Leiomyosarcoma - metabolism
Medical prognosis
Myocytes, Smooth Muscle - metabolism
Optical microscopes
Pathophysiology
Phytochemicals
Sarcoma
Smooth muscle
Soft tissue sarcoma
Soft tissues
Spheroids
Stains
Strawberries
strawberry
Tumors
Uterine Neoplasms - drug therapy
Uterine Neoplasms - metabolism
Uterus
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Summary:Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 µg/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson's trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu15112557