Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4',6-Diamidino-2-phenylindole-Staining

Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based as...

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Bibliographic Details
Published in:International Journal of Cell Biology 2014, Vol.2014 (2014), p.154-160
Main Authors: Oliveira, Tatiane, Figueiredo, Camila A., Brito, Carlos, Stavroullakis, Alexander, Prakki, Anuradha, Da Silva Velozo, Eudes, Nogueira-Filho, Getulio
Format: Article
Language:English
Subjects:
Allium cepa
Apoptosis
Cell growth
Cell interaction
Colleges & universities
Deoxyribonucleic acid
DNA
E coli
Escherichia coli
Health aspects
Herbal medicine
Identification and classification
Lipopolysaccharides
Onions
Osteoblasts
Porphyromonas gingivalis
Proteins
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Summary:Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO2. Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells.
ISSN:1687-8876
1687-8884
DOI:10.1155/2014/535789