Seminal plasma miR-192a: a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair

This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA (miR)-192a levels in seminal plasma and testicular tissue were evaluated by quantitative...

Full description

Saved in:
Bibliographic Details
Published in:Asian journal of andrology 2018-07, Vol.20 (4), p.396-399
Main Authors: Zhi, Er-Lei, Liang, Guo-Qing, Li, Peng, Chen, Hui-Xing, Tian, Ru-Hui, Xu, Peng, Li, Zheng
Format: Article
Language:English
Subjects:
Apoptosis
Biomarkers
Gene expression
Histology
Meta-analysis
MicroRNAs
miR-192a
nonobstructive azoospermia
seminal plasma
varicocelectomy
Original
Plasma
Proteins
RNA
Sperm
Spermatogenesis
Surgery
Systematic review
Ultrasonic imaging
Varicocele
Veins & arteries
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA (miR)-192a levels in seminal plasma and testicular tissue were evaluated by quantitative real-time polymerase chain reaction from 60 men with nonobstructive azoospermia and varicoceles (Group A: 27 men with spermatozoa found in the ejaculate after surgery; Group B: 33 men without spermatozoa found in the ejaculate after surgery) and 30 controls. The seminal plasma and testicular tissue miR-192a levels were higher in Group B than in Group A and the controls (P < 0.001), and there was no significant difference between Group A and the controls (P > 0.05). Apoptosis and proliferation assays with miR mimics and inhibitors showed that miR-192a induced GC-2 cell apoptosis through the activation of Caspase-3 protein. Thus, seminal plasma miR-192a appears to be a potential marker for successfully indicating spermatozoa in the ejaculate following microsurgical varicocelectomy in men with nonobstructive azoospermia and varicoceles. Seminal plasma miR-192a may be a useful clinical marker for prescreening to determine which patients with nonobstructive azoospermia and varicoceles would benefit from varicocelectomy.
ISSN:1008-682X
1745-7262
DOI:10.4103/aja.aja_8_18