Synergistic inhibition of Haemonchus contortus exsheathment by flavonoid monomers and condensed tannins

• Published in: International journal for parasitology -- drugs and drug resistance 2015-12, Vol.5 (3), p.127-134
• Main Authors: Klongsiriwet, Chaweewan, Quijada, Jessica, Williams, Andrew R., Mueller-Harvey, Irene, Williamson, Elizabeth M., Hoste, Hervé
• Format: Article
• Language: English
• Subjects: Animals; Flavan-3-ols; Flavonoids - chemistry; Flavonoids - pharmacology; Haemonchus - drug effects; Haemonchus - physiology; Larva - drug effects; Larva - physiology; Life Sciences; Luteolin; Mean degree of polymerisation; Molecular Structure; Nematodes; Plants - chemistry; Proanthocyanidins; Proanthocyanidins - chemistry; Proanthocyanidins - pharmacology; Procyanidins; Prodelphinidins; Quercetin
• ISSN: 2211-3207; 2211-3207
• DOI: 10.1016/j.ijpddr.2015.06.001

This study investigated the separate and combined anthelmintic (AH) effects of different phenolic compounds, including condensed tannins and flavonoids, all of which are known to occur in willow leaves, a potentially valuable dry season feed. A range of contrasting model tannins, which span the whole range of willow tannins, were isolated from tilia flowers, goat willow leaves, black currant leaves and red currant leaves. All together, the tested compounds represented the major tannin types (procyanidins and prodelphinidins) and flavonoid types (flavonols, flavones and flavanones). The larval exsheathment inhibition assay (LEIA) was used to assess their in vitro effects on Haemonchus contortus third stage larvae. Arbutin, vanillic acid, and taxifolin proved to be ineffective whereas naringenin, quercetin and luteolin were highly effective at 250 μM concentrations. Procyanidin (PC) tannins tended to be less active than prodelphinidin tannins (PD). Experiments with combinations of tannins and quercetin or luteolin revealed for the first time the existence of synergistic AH effects between tannins and flavonoid monomers. They also provided evidence that synergistic effects appear to occur at slightly lower concentrations of PC than PD. This suggests that the AH activity of condensed tannins can be significantly enhanced by the addition of quercetin or luteolin. This information may prove useful for plant breeding or selection and for designing optimal feed mixtures. [Display omitted] •Tannins and flavonoids showed anti-parasitic effects against Haemonchus contortus.•This is the first report of their synergistic effects on larval exsheathment.•Flavonoids enhanced especially the anthelmintic activities of procyanidin tannins.