Astragaloside IV Suppresses Hepatic Proliferation in Regenerating Rat Liver after 70% Partial Hepatectomy via Down-Regulation of Cell Cycle Pathway and DNA Replication

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-05, Vol.26 (10), p.2895
• Main Authors: Lee, Gyeong-Seok, Jeong, Hee-Yeon, Yang, Hyeon-Gung, Seo, Young-Ran, Jung, Eui-Gil, Lee, Yong-Seok, Nam, Kung-Woo, Kim, Wan-Jong
• Format: Article
• Language: English
• Subjects: AKT protein; Animals; Annotations; Astragali Radix; astragaloside IV; Astragalus membranaceus; Cancer; Cell cycle; Cell Cycle - drug effects; Cell division; Cell Proliferation - drug effects; Chinese medicine; Cholesterol; Cyclin D1; Cyclin D1 - metabolism; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; DNA; DNA biosynthesis; DNA Replication - drug effects; Down-Regulation - drug effects; Gene expression; Gene Expression Regulation - drug effects; Gene Regulatory Networks - drug effects; Genes; Growth factors; Hepatectomy; Hepatocyte growth factor; Hepatocyte Growth Factor - metabolism; Herbal medicine; huang qi; Hypoxia; Liver; Liver - cytology; Liver - drug effects; Liver - surgery; liver regeneration; Liver Regeneration - drug effects; Male; MAP kinase; Medicinal plants; Molecular Sequence Annotation; Oral administration; Proteins; Rats; Rats, Sprague-Dawley; Replication; RNA, Messenger - genetics; RNA, Messenger - metabolism; Saponins - chemistry; Saponins - pharmacology; Sequence Analysis, RNA; Signal transduction; Transforming Growth Factor beta1 - metabolism; Triterpenes - chemistry; Triterpenes - pharmacology
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26102895

Astragaloside IV (AS-IV) is one of the major bio-active ingredients of huang qi which is the dried root of (a traditional Chinese medicinal plant). The pharmacological effects of AS-IV, including anti-oxidative, anti-cancer, and anti-diabetic effects have been actively studied, however, the effects of AS-IV on liver regeneration have not yet been fully described. Thus, the aim of this study was to explore the effects of AS-IV on regenerating liver after 70% partial hepatectomy (PHx) in rats. Differentially expressed mRNAs, proliferative marker and growth factors were analyzed. AS-IV (10 mg/kg) was administrated orally 2 h before surgery. We found 20 core genes showed effects of AS-IV, many of which were involved with functions related to DNA replication during cell division. AS-IV down-regulates MAPK signaling, PI3/Akt signaling, and cell cycle pathway. Hepatocyte growth factor (HGF) and cyclin D1 expression were also decreased by AS-IV administration. Transforming growth factor β1 (TGFβ1, growth regulation signal) was slightly increased. In short, AS-IV down-regulated proliferative signals and genes related to DNA replication. In conclusion, AS-IV showed anti-proliferative activity in regenerating liver tissue after 70% PHx.