An approach to an inhibition electronic tongue to detect on-line organophosphorus insecticides using a computer controlled multi-commuted flow system

An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Mu...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2011-04, Vol.11 (4), p.3791-3802
Main Authors: Alonso, Gustavo A, Dominguez, Rocio B, Marty, Jean-Louis, Muñoz, Roberto
Format: Article
Language:English
Subjects:
Acetylcholinesterase - chemistry
Animals
Automation
Azinphosmethyl - analogs & derivatives
Azinphosmethyl - analysis
Benzenesulfonates - analysis
Biosensing Techniques - instrumentation
biosensor
Biosensors
Chlorpyrifos - analogs & derivatives
Chlorpyrifos - analysis
Drosophila - chemistry
Electrodes
enzyme inhibition
Enzyme Inhibitors
Enzymes
flow system
Insecticides
Insecticides - analysis
Laboratories
multi-commuted
organo-phosphorus insecticides
Personal computers
Polyvinyl alcohol
Potentiometry - methods
potentiostat
Reagents
Sensors
Software
Valves
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Summary:An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Multi-commuted Flow Analysis system (MCFA) was selected and developed to carry out assays with an improved inhibition method to detect the insecticides chlorpyrifos oxon (CPO), chlorfenvinfos (CFV) and azinphos methyl-oxon (AZMO). The system manifold comprised a peristaltic pump, a set of seven electronic valves controlled by a personal computer electronic interface and software based on LabView® to control the sample dilutions into the cell. The inhibition method consists in the injection of the insecticide when the enzyme activity has reached the plateau of the current; with this method the incubation time is avoided. A potentiostat was developed to measure the response from the enzymatic biosensor. Low limits of detection of 10 nM for CPO, CFV, and AZMO were achieved.
ISSN:1424-8220
1424-8220
DOI:10.3390/s110403791