Candida parapsilosis Cell Wall Proteome Characterization and Effectiveness against Hematogenously Disseminated Candidiasis in a Murine Model

poses huge treatment challenges in the clinical settings of South Africa, and often causes infections among immunocompromised patients and underweight neonates. Cell wall proteins have been known to play vital roles in fungal pathogenesis, as these are the first points of contact toward environments...

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Bibliographic Details
Published in:Vaccines (Basel) 2023-03, Vol.11 (3), p.674
Main Authors: Gong, Xiaolong, Srivastava, Vartika, Naicker, Previn, Khan, Amber, Ahmad, Aijaz
Format: Article
Language:English
Subjects:
Ammonium
Animal models
Antifungal agents
Antigens
BALB/c mice
Bicarbonates
Biomarkers
Candida parapsilosis
Candidiasis
cell wall proteins
Cell walls
Fungi
Fungicides
Glycerol
Immune system
Immunization
Immunocompromised hosts
Immunogenicity
Immunosuppressive agents
Laboratories
Lethal dose
Medical equipment
Multidrug resistance
Neonates
Nosocomial infections
Pathogenesis
Pathogens
Peptides
Proteinase
Proteins
Proteomes
Secretions
Survival
Underweight
Vaccine development
Vaccines
Yeasts
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Summary:poses huge treatment challenges in the clinical settings of South Africa, and often causes infections among immunocompromised patients and underweight neonates. Cell wall proteins have been known to play vital roles in fungal pathogenesis, as these are the first points of contact toward environments, the host, and the immune system. This study characterized the cell wall immunodominant proteins of pathogenic yeast and evaluated their protective effects in mice, which could add value in vaccine development against the rising infections. Among different clinical strains, the most pathogenic and multidrug-resistant isolate was selected based on their susceptibility towards antifungal drugs, proteinase, and phospholipase secretions. Cell wall antigens were prepared by β-mercaptoethanol/ammonium bicarbonate extraction from selected strains. Antigenic proteins were identified using LC-MS/MS, where 933 proteins were found, with 34 being immunodominant. The protective effect of the cell wall immunodominant proteins was observed by immunizing BALB/c mice with cell wall protein extracts. After the immunization and booster, the BALC/c mice were challenged with a lethal dose of . In vivo results demonstrated increased survival rates and lower fungal burden in vital organs in the immunized mice compared to the unimmunized mice, thereby confirming the immunogenic property of cell wall-associated proteins of . Therefore, these results advocated the potential of these cell wall proteins to act as biomarkers for the development of diagnostic assays and/or vaccines against infections caused by .
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines11030674