Impact of Intrahost NS5 Nucleotide Variations on Dengue Virus Replication

Due to the nature of RNA viruses, their high mutation rates produce a population of closely related but genetically diverse viruses, termed quasispecies. To determine the role of quasispecies in DENV disease severity, 22 isolates (10 from mild cases, 12 from fatal cases) were obtained, amplified, an...

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Bibliographic Details
Published in:Frontiers in microbiology 2022-07, Vol.13, p.894200-894200
Main Authors: Cheng, Dayna, Huang, Sheng-Wen, Chin, Wei-Xin, Hung, Su-Jhen, Tsai, Huey-Pin, Chu, Justin Jang Hann, Chao, Chiao-Hsuan, Wang, Jen-Ren
Format: Article
Language:English
Subjects:
dengue virus
disease severity
intrahost variations on dengue replication
Microbiology
next-generation sequencing
quasispecies
virulence
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Summary:Due to the nature of RNA viruses, their high mutation rates produce a population of closely related but genetically diverse viruses, termed quasispecies. To determine the role of quasispecies in DENV disease severity, 22 isolates (10 from mild cases, 12 from fatal cases) were obtained, amplified, and sequenced with Next Generation Sequencing using the Illumina MiSeq platform. Using variation calling, unique wildtype nucleotide positions were selected and analyzed for variant nucleotides between mild and fatal cases. The analysis of variant nucleotides between mild and fatal cases showed 6 positions with a significant difference of p  
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.894200