Proteomic analysis of fipronil-induced molecular defects in spermatozoa

The phenylpyrazole insecticide fipronil has wide-ranging applications from agriculture to public health to control undesirable organisms. However, several studies have reported the residual environmental hazards of fipronil and demonstrated its harmful effects even in mammalian reproduction. Therefo...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2024-04, Vol.14 (1), p.7668-7668, Article 7668
Main Authors: Bae, Jeong-Won, Kwon, Woo-Sung
Format: Article
Language:English
Subjects:
631/337/475
692/499
704/172/4081
Acrosome reaction
Cytoskeleton
Differentially expressed proteins
Environmental hazards
Fertility
Fipronil
Humanities and Social Sciences
Infertility
Insecticides
Male infertility
Mode of action
multidisciplinary
Pesticide-affected spermatozoa
Pesticides
Phenylpyrazole
Proteins
Proteomics
Public health
Science
Science (multidisciplinary)
Sperm
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The phenylpyrazole insecticide fipronil has wide-ranging applications from agriculture to public health to control undesirable organisms. However, several studies have reported the residual environmental hazards of fipronil and demonstrated its harmful effects even in mammalian reproduction. Therefore, this study was conducted to demonstrate the mode of action of fipronil on mouse spermatozoa. We treated fipronil to spermatozoa and performed comprehensive function evaluations. Moreover, proteomic analyses were conducted to identify the alteration of protein expression levels in spermatozoa. Most of sperm motility and kinematic parameters and intracellular ATP levels were diminished, and the spontaneous acrosome reaction was promoted after treatment with fipronil. Proteomic analyses revealed altered expression levels of 14 proteins after treatment. These proteins have been reported to be associated with sperm-specific pathways, prominently the cytoskeleton of the sperm, “9 + 2” axoneme composition, metabolism, and fertility. Collectively, our results showed that fipronil alters sperm functional-related proteins and therefore influences male fertility. This study elucidates the possible reproductive toxic hazards associated with male infertility through aberrant suppression of sperm proteins.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-57876-4