Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9

NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Hu...

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Bibliographic Details
Published in:Stem cell research 2023-03, Vol.67, p.103022-103022, Article 103022
Main Authors: Guo, Ruirui, Yuan, Simin, Li, Bing, Wang, Jichang, Sun, Chuanbo
Format: Article
Language:English
Subjects:
Cell Differentiation
Cell Line
CRISPR-Cas Systems
Embryonic Stem Cells - metabolism
Germ Cells - metabolism
Human Embryonic Stem Cells - metabolism
Humans
RNA-Binding Proteins - genetics
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Summary:NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Human primordial germ cell-like cells (hPGCLCs) generated from pluripotent stem cells is an excellent alternatives in human germ cell-related studies. This hESC line with an mCherry knock-in at the site before NANOS3′s stop codon serves as a useful tool to learn human PGC specification.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2023.103022