Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt

Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (...

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Published in:Marine drugs 2022-10, Vol.20 (10), p.646
Main Authors: Miao, Qin, Li, Qing, Tan, Wenqiang, Mi, Yingqi, Ma, Bing, Zhang, Jingjing, Guo, Zhanyong
Format: Article
Language:English
Subjects:
Acids
Analytical methods
Anions
anticoagulant activity
Anticoagulants
antioxidant activity
Antioxidant properties
Antioxidants
Biological activity
Cations
Economic benefits
Glucosamine
glucosamine hydrochloride
glucosamine-heparin salt
Hemodialysis
Heparin
heparin sodium
Hydrogen
Investigations
Ion exchange
Ions
Magnetic resonance spectroscopy
Molecular weight
NMR
NMR spectroscopy
Nuclear magnetic resonance
Radicals
Salt
Salts
Scavenging
Sodium
Spectrum analysis
Sugar
Vibration
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Summary:Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (GHS) was primarily synthesized with positively charged glucosamine hydrochloride (GAH) and negatively charged heparin sodium (Heps) by ion exchange method. Then, the detailed structural information of the GHS was characterized by FTIR, 1H NMR spectroscopy and ICP-MS. In addition, its anticoagulant potency and antioxidant properties were evaluated, respectively. The results demonstrated that GHS salt achieved enhanced antioxidant activities, including 98.78% of O2•− radical scavenging activity, 91.23% of •OH radical scavenging rate and 66.49% of DPPH radical scavenging capacity at 1.6 mg/mL, severally. Meanwhile, anticoagulant potency (ATTP) of GHS strengthened from 153.10 ± 17.14 to 180.03 ± 6.02 at 0.75 μmol/L. Thus, introducing cationic glucosamine residues into GHS could improve its anticoagulant activity. The findings suggest that GHS product with a small amount of inorganic ions can greatly abate the prime cost of antioxidants and anticoagulants, and has significant economic benefits and practical significance.
ISSN:1660-3397
1660-3397
DOI:10.3390/md20100646