Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy

• Published in: Photodiagnosis and photodynamic therapy 2025-02, Vol.51, p.104431, Article 104431
• Main Authors: Chen, Xiuqin, Chen, Guizhi, Cao, Sitong, Ye, Ruoxin, Qiu, Ruoyi, Yang, Xiangyu, Peng, Yiru, Sun, Hong
• Format: Article
• Language: English
• Subjects: Lysosomal targeting; Photodynamic therapy; Phthalocyanine; Pyrrolidine ketone; Two-photon fluorescence imaging
• ISSN: 1572-1000; 1873-1597; 1873-1597
• DOI: 10.1016/j.pdpdt.2024.104431

•Benzopyrrolidinyl-substituted silicon phthalocyanine (Py-SiPc) was synthesized and characterized.•DSPE@Py-SiPc was prepared by loading Py-SiPc into DSPE-PEG2000.•DSPE@Py-SiPc induced lysosomal cell death under laser irradiation.•DSPE@Py-SiPc was a lysosomal labeling two-photon probe. Lysosomes are pivotal in diverse physiological phenomena, encompassing autophagy, apoptosis, and cellular senescence. The demand for precise tumors treatment has led to the development of specific lysosome-targeting probes capable of elucidating lysosomal dynamics and facilitating targeted cell death. In this research, we report the synthesis and characterization of a novel benzopyrrolidinyl-substituted silicon phthalocyanine (Py-SiPc), designed for selective lysosome labeling and Fluorescence imaging-guided in vitro photodynamic therapy. Furthermore, we encapsulated Py-SiPc within a biocompatible nanocarrier, dipalmitoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE), to create water-soluble nanoparticles (DSPE@Py-SiPc). These nanoparticles exhibit exceptional lysosome labeling capabilities, as evidenced by bioimaging techniques. Upon exposure to laser irradiation, DSPE@Py-SiPc efficiently induces the production of reactive oxygen species, impairing lysosomal function and triggering lysosomal-mediated cell death. The DSPE@Py-SiPc system emerges as a promising photosensitizer. [Display omitted]