3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties

Biomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, the use of such ma...

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Bibliographic Details
Published in:iScience 2022-09, Vol.25 (9), p.104947, Article 104947
Main Authors: Theus, Andrea S., Ning, Liqun, Kabboul, Gabriella, Hwang, Boeun, Tomov, Martin L., LaRock, Christopher N., Bauser-Heaton, Holly, Mahmoudi, Morteza, Serpooshan, Vahid
Format: Article
Language:English
Subjects:
Biomaterials
Nanoparticles
Tissue engineering
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Summary:Biomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, the use of such materials in bioprinting-based approaches of scaffold fabrication has not been examined. This study introduces a new generation of bacteriostatic gelatin methacryloyl (GelMA)-based bioinks, incorporated with varying doses of antibacterial superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-laden GelMA scaffolds showed significant resistance against the Staphylococcus aureus growth, while providing a contrast in magnetic resonance imaging. We simulated the bacterial contamination of cellular 3D GelMA scaffolds in vitro and demonstrated the significant effect of functionalized scaffolds in inhibiting bacterial growth, while maintaining cell viability and growth. Together, these results present a new promising class of functionalized bioinks to 3D bioprint tissue-engineered scaffold with markedly enhanced properties for the use in a variety of in vitro and clinical applications. [Display omitted] •Functionalized bioinks with bacteriostatic properties are developed and thoroughly characterized•The 200 μg/mL group yielded an optimal balance of printed scaffold properties•Incorporating nanoparticle also enabled noninvasive imaging of the bioprinted scaffold Biomaterials; Nanoparticles; Tissue engineering
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2022.104947