The Effect of Mutation in Lipopolysaccharide Biosynthesis on Bacterial Fitness

This paper presents the genome sequence of a mutant strain ( 4351) and the effect of mutation in lipopolysaccharide biosynthesis on bacterial fitness. Lipopolysaccharides are the major component of the outer leaflet of the Gram-negative outer membrane. We report here a frameshift mutation of the gen...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2022-10, Vol.11 (20), p.3249
Main Authors: Nagy, Laura, Urbán, Péter, Makszin, Lilla, Sándor, Viktor, Kilár, Anikó, Ábrahám, Hajnalka, Albert, Beáta, Kocsis, Béla, Kilár, Ferenc
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteria
bacterial genome
Bacterial Proteins - genetics
Biosynthesis
Carbohydrate Epimerases - genetics
Cell division
Cephalexin - pharmacology
endotoxin
Enzymes
Erythromycin
Erythromycin - pharmacology
Frameshift Mutation
Gene expression
Genetic aspects
Genetic Fitness
Genome, Bacterial
Genomes
Genomic analysis
Health aspects
Heptose
Kinases
Lipids
lipopolysaccharide biosynthesis
Lipopolysaccharides
Lipopolysaccharides - genetics
Microscopy
Mutation
Mutation (Biology)
Nucleotide sequence
Phosphatase
Phosphates
Physiological aspects
Reproductive fitness
Shigella sonnei - drug effects
Shigella sonnei - genetics
Shigella sonnei 4351
Software
Virulence
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Summary:This paper presents the genome sequence of a mutant strain ( 4351) and the effect of mutation in lipopolysaccharide biosynthesis on bacterial fitness. Lipopolysaccharides are the major component of the outer leaflet of the Gram-negative outer membrane. We report here a frameshift mutation of the gene in the genome of 4351. The mutation results in a lack of epimerization of the core heptose while we also found increased thermosensitivity, abnormal cell division, and increased susceptibility to erythromycin and cefalexin compared to the 4303. Comparative genomic analysis supplemented with structural data helps us to understand the effect of specific mutations on the virulence of the bacteria and may provide an opportunity to study the effect of short lipopolysaccharides.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells11203249