Review: Advanced Atomic Force Microscopy Modes for Biomedical Research

Visualization of biomedical samples in their native environments at the microscopic scale is crucial for studying fundamental principles and discovering biomedical systems with complex interaction. The study of dynamic biological processes requires a microscope system with multiple modalities, high...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-12, Vol.12 (12), p.1116
Main Authors: Xia, Fangzhou, Youcef-Toumi, Kamal
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biological activity
Biomedical Research
Care and treatment
Diagnosis
high-speed imaging
Imaging
Innovations
Light
material property mapping
mechanobiology
Medical research
Medicine, Experimental
Microorganisms
Microscopes
Microscopy
Microscopy, Atomic Force - methods
nano-manipulation
nanotechnology
Nanotechnology - methods
Review
Spectroscopy
Technology application
Temporal resolution
Topography
Virus diseases
Viruses
Visualization
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Summary:Visualization of biomedical samples in their native environments at the microscopic scale is crucial for studying fundamental principles and discovering biomedical systems with complex interaction. The study of dynamic biological processes requires a microscope system with multiple modalities, high spatial/temporal resolution, large imaging ranges, versatile imaging environments and ideally in-situ manipulation capabilities. Recent development of new Atomic Force Microscopy (AFM) capabilities has made it such a powerful tool for biological and biomedical research. This review introduces novel AFM functionalities including high-speed imaging for dynamic process visualization, mechanobiology with force spectroscopy, molecular species characterization, and AFM nano-manipulation. These capabilities enable many new possibilities for novel scientific research and allow scientists to observe and explore processes at the nanoscale like never before. Selected application examples from recent studies are provided to demonstrate the effectiveness of these AFM techniques.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12121116