Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)

In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2 Uma be...

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Published in:Brazilian archives of biology and technology 2006-11, Vol.49 (6), p.881-888
Main Authors: Vieira, Felipe Almeida(Universidade Estadual Norte Fluminense Darcy Ribeiro Laboratório de fisiologia e Bioquímica de Microrganismos), Cunha, Maura da(Universidade Estadual Norte Fluminense Darcy Ribeiro Centro de Biologia e Biotecnologia Laboratório de Biologia Celular e Tecidual), Klein, Denise Espellet(Universidade Estadual Norte Fluminense Darcy Ribeiro Centro de Biologia e Biotecnologia Laboratório de Biologia Celular e Tecidual), Carvalho, André de Oliveira(Universidade Estadual Norte Fluminense Darcy Ribeiro Laboratório de fisiologia e Bioquímica de Microrganismos), Gomes, Valdirene Moreira(Universidade Estadual Norte Fluminense Darcy Ribeiro Laboratório de fisiologia e Bioquímica de Microrganismos)
Format: Article
Language:English
Subjects:
beta-1,3-glucanase
chitinases
PR protein
secretory structure
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Summary:In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2 Uma beta-1,3-glucanase foi purificada a partir da secreção de Simira glaziovii, através de vários processos cromatográficos, tendo a análise do perfil protéico acompanhado de SDS-PAGE. A enzima purificada apresentou uma massa molecular estimada de 35 kDa. O pH ótimo obtido para a enzima foi de 5,2.
ISSN:1516-8913
1516-8913
1678-4324
DOI:10.1590/S1516-89132006000700004