Rheumatoid arthritis-associated microRNA-155 targets SOCS1 and upregulates TNF-α and IL-1β in PBMCs

• Published in: International journal of molecular sciences 2013-12, Vol.14 (12), p.23910-23921
• Main Authors: Li, Xiaochuan, Tian, Feng, Wang, Fei
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Adult; Arthritis, Rheumatoid - genetics; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; Base Sequence; Female; Humans; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Leukocytes, Mononuclear - metabolism; Lipopolysaccharides - toxicity; Male; MicroRNAs - antagonists & inhibitors; MicroRNAs - metabolism; Middle Aged; miR-155; Oligonucleotides, Antisense - metabolism; rheumatoid arthritis; RNA, Messenger - metabolism; SOCS1; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling Proteins - antagonists & inhibitors; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; TNF-α and IL-1β; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Up-Regulation - drug effects; Young Adult
• ISSN: 1422-0067; 1422-0067
• DOI: 10.3390/ijms141223910

miR-155 plays a crucial role in proinflammatory activation. This study was carried out to assess the association of abnormal expression of miR-155 in peripheral blood of patients with Rheumatoid arthritis with the expression of TNF-α and IL-1β. Release of TNF-α and IL-1β, and expression of miR-155 were determined in RA peripheral blood or peripheral blood macrophages, followed by correlation analysis of the cytokines release and miR-155 expression. Furthermore, in vitro studies indicate that miR-155 inhibited the expression of SOCS1. Our results suggest that there is a correlation between the high-level expression of miR-155 and the enhanced expression of TNF-α and IL-1β. miR-155 targets and suppresses the expression of SOCS1, and the decrease of SOCS1 may lead to the upregulation of TNF-α and IL-1β.