Transcriptome Analysis of Clinopodium chinense (Benth.) O. Kuntze and Identification of Genes Involved in Triterpenoid Saponin Biosynthesis

(Benth.) O. Kuntze ( ) is an important herb in traditional Chinese medicine. Triterpenoid saponins are a major class of active compounds in with broad pharmacological activities and hemostatic, antitumor, and anti-hyperglycemic effects. To identify genes involved in triterpenoid saponin biosynthesis...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-05, Vol.20 (11), p.2643
Main Authors: Shi, Yuanyuan, Zhang, Shengxiang, Peng, Daiyin, Wang, Chenkai, Zhao, Derui, Ma, Kelong, Wu, Jiawen, Huang, Luqi
Format: Article
Language:English
Subjects:
Annotations
Antitumor activity
Bioinformatics
Biosynthesis
Clinopodium
Clinopodium chinense (Benth.) O. Kuntze
Coding
differentially expressed genes
Enzymes
Gene expression
Genes
Genomes
Genomics
Herbal medicine
Information processing
Laboratories
Leaves
Medicine
Metabolism
Metabolites
Pharmacology
Proteins
RNA sequencing
Saponins
Secondary metabolites
Software
Transcription factors
transcriptome
triterpenoid saponin biosynthesis
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Summary:(Benth.) O. Kuntze ( ) is an important herb in traditional Chinese medicine. Triterpenoid saponins are a major class of active compounds in with broad pharmacological activities and hemostatic, antitumor, and anti-hyperglycemic effects. To identify genes involved in triterpenoid saponin biosynthesis, transcriptomic analyses of leaves, stems, and roots from were performed. A total of 135,968 unigenes were obtained by assembling the leaf, stem, and root transcripts, of which 102,154 were annotated in public databases. Differentially expressed genes were determined based on expression profile analysis and analyzed for differential expression of unique genes related to triterpenoid saponin biosynthesis. Multiple unigenes encoding crucial enzymes or transcription factors involved in triterpenoid saponin synthesis were identified and analyzed. The expression levels of unigenes encoding enzymes were experimentally validated using quantitative real-time PCR. This study greatly broadens the public transcriptome database for this species and provides a valuable resource for identifying candidate genes involved in the biosynthesis of triterpenoid saponins and other secondary metabolites.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20112643