Microsphere incorporation as a strategy to tune the biological performance of bioinks

Although alginate is widely used as a matrix in the formulation of cell-laden inks, this polymer often requires laborious processing strategies due to its lack of cell adhesion moieties. The main objective of the present work was to explore the incorporation of microspheres into alginate-based bioin...

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Bibliographic Details
Published in:Journal of tissue engineering 2022, Vol.13, p.20417314221119895-20417314221119895
Main Authors: Bonany, Mar, del-Mazo-Barbara, Laura, Espanol, Montserrat, Ginebra, Maria-Pau
Format: Article
Language:English
Subjects:
Adhesion
Alginates
Alginic acid
Calcium ions
Cell adhesion
Cell adhesion & migration
Cell culture
Cell differentiation
Cell migration
Cell proliferation
Differentiation (biology)
Fabrication
Hydroxyapatite
Inks
Mechanical properties
Microspheres
Nanoparticles
Original
Polymers
Rheological properties
Stiffness
Supplements
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Summary:Although alginate is widely used as a matrix in the formulation of cell-laden inks, this polymer often requires laborious processing strategies due to its lack of cell adhesion moieties. The main objective of the present work was to explore the incorporation of microspheres into alginate-based bioinks as a simple and tuneable way to solve the cell adhesion problems, while adding extra biological functionality and improving their mechanical properties. To this end, three types of microspheres with different mineral contents (i.e. gelatine with 0% of hydroxyapatite, gelatine with 25 wt% of hydroxyapatite nanoparticles and 100 wt% of calcium -deficient hydroxyapatite) were synthesised and incorporated into the formulation of cell-laden inks. The results showed that the addition of microspheres generally improved the rheological properties of the ink, favoured cell proliferation and positively affected osteogenic cell differentiation. Furthermore, this differentiation was found to be influenced by the type of microsphere and the ability of the cells to migrate towards them, which was highly dependent on the stiffness of the bioink. In this regard, Ca2+ supplementation in the cell culture medium had a pronounced effect on the relaxation of the stiffness of these cell-loaded inks, influencing the overall cell performance. In conclusion, we have developed a powerful and tuneable strategy for the fabrication of alginate-based bioinks with enhanced biological characteristics by incorporating microspheres into the initial ink formulation.
ISSN:2041-7314
2041-7314
DOI:10.1177/20417314221119895