Bhargavaea beijingensis a promising tool for bio-cementation, soil improvement, and mercury removal

• Published in: Scientific reports 2024-10, Vol.14 (1), p.23976-16, Article 23976
• Main Authors: Gadhvi, Megha S., Javia, Bhumi M., Vyas, Suhas J., Patel, Rajesh, Dudhagara, Dushyant R.
• Format: Article
• Language: English
• Subjects: 631/1647; 631/326; 631/61; 639/166; 704/172; Bacteria; Bhargavaea beijingensis; Bio-cementation; Biodegradation, Environmental; Calcareous soils; Calcite; Calcite precipitation; Calcium Carbonate - chemistry; Calcium Carbonate - metabolism; Chemical precipitation; Compressive Strength; Enzymes; Heavy metal removal; Heavy metals; Humanities and Social Sciences; Mercuric chloride; Mercury; Mercury - metabolism; Metals, Heavy - metabolism; MICP; Mining; multidisciplinary; Remediation; Science; Science (multidisciplinary); Soil - chemistry; Soil improvement; Soil Microbiology; Soil microorganisms; Soil Pollutants - metabolism; Soil remediation; Urease; Urease - metabolism; Ureolytic bacteria
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-75019-7

Microbially Induced Calcite Precipitation (MICP) has emerged as a promising technique for bio-cementation, soil improvement, and heavy metal remediation. This study explores the potential of Bhargavaea beijingensis , a urease-producing bacterium, for these applications. Six ureolytic bacteria were isolated from calcareous bricks mine soil and screened for urease and calcite production. B. beijingensis exhibited the highest urease activity and calcite precipitation. Urease activity, calcite precipitation, sand solidification, heavy metal removal efficiency, and compressive strength were evaluated. It showed significant heavy metal removal efficiency, particularly highest for HgCl 2 . Mortar blocks treated with B. beijingensis or its crude enzyme exhibited improved compressive strength, suggesting its potential for bio-cementation. Crack remediation tests demonstrated successful crack healing in mortar blocks using the bacterium or its enzyme. This study identifies B. beijingensis as a novel and promising MICP agent with potential applications in bio-cementation, soil improvement, and heavy metal remediation. Hence, B. beijingensis diversified abilities prove superior performance compared to commonly used strains like Bacillus subtilis and Shewanella putrefaciens in bio-cementation applications. Its high urease activity, calcite precipitation, and heavy metal removal abilities make it a valuable candidate for sustainable and eco-friendly solutions in various fields.