Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan

• Published in: Acta veterinaria scandinavica 2011-10, Vol.53 (1), p.52-52, Article 52
• Main Authors: Asai, Tetsuo, Masani, Kaori, Sato, Chizuru, Hiki, Mototaka, Usui, Masaru, Baba, Kotaro, Ozawa, Manao, Harada, Kazuki, Aoki, Hiroshi, Sawada, Takuo
• Format: Article
• Language: English
• Subjects: AmpC β-lactamase; Animals; Bacteria; beta-Lactamases - biosynthesis; beta-Lactamases - genetics; Brief Communication; Cattle; Cattle - microbiology; Cephaloridine; cephalosporin resistance; Cephalosporin Resistance - genetics; Cephalosporins; DNA Primers; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Escherichia coli Proteins - genetics; extended spectrum β-lactamase; Food Chain; Food Microbiology; food-producing animals; Genes; Genetic aspects; Health aspects; Japan - epidemiology; Meat - microbiology; Microbiology; Moxalactam; phylogenetic grouping; Phylogeny; Polymerase Chain Reaction; Poultry; Poultry - microbiology; Swine - microbiology
• ISSN: 1751-0147; 0044-605X; 1751-0147
• DOI: 10.1186/1751-0147-53-52

A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced bla(CTX-M-2), while the nine poultry isolates produced bla(CTX-M-25) (4), bla(SHV-2) (3), bla(CTX-M-15) (1) and bla(CTX-M-2) (1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in E. coli from diseased animals in Japan.