Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization

Type VI secretion systems (T6SS), recently described in hypervirulent K. pneumoniae (hvKp) strains , are involved in bacterial warfare but their role in classical clinical strains (cKp) has been little investigated . In silico analysis indicated the presence of T6SS clusters (from zero to four), irr...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-10, Vol.12 (1), p.16968-16968, Article 16968
Main Authors: Merciecca, Thomas, Bornes, Stéphanie, Nakusi, Laurence, Theil, Sébastien, Rendueles, Olaya, Forestier, Christiane, Miquel, Sylvie
Format: Article
Language:English
Subjects:
631/326/1320
631/326/2565/2134
Animals
Bacteriology
Biodiversity and Ecology
Cell membranes
Comparative analysis
Competition
E coli
Environmental Sciences
Escherichia coli - genetics
Escherichia coli - metabolism
Gastrointestinal tract
Gastrointestinal Tract - metabolism
Genes
Genomes
Genomics
Humanities and Social Sciences
Klebsiella pneumoniae
Life Sciences
Membrane permeability
Mice
Microbiology and Parasitology
Microbiota
multidisciplinary
Nosocomial infections
Pathogenicity
Pathogenicity islands
Pathogens
Periplasm
Phospholipases - metabolism
Proteins
Science
Science (multidisciplinary)
Secretion
Strains (organisms)
Type VI Secretion Systems - genetics
Type VI Secretion Systems - metabolism
Type VII Secretion Systems - metabolism
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Type VI secretion systems (T6SS), recently described in hypervirulent K. pneumoniae (hvKp) strains , are involved in bacterial warfare but their role in classical clinical strains (cKp) has been little investigated . In silico analysis indicated the presence of T6SS clusters (from zero to four), irrespective of the strains origin or virulence, with a high prevalence in the K. pneumoniae species (98%). In the strain CH1157, two T6SS-apparented pathogenicity islands were detected, T6SS-1 and -2, harboring a phospholipase-encoding gene ( tle1 ) and a potential new effector-encoding gene named tke (Type VI Klebsiella effector). Tle1 expression in Escherichia coli periplasm affected cell membrane permeability. T6SS-1 isogenic mutants colonized the highest gastrointestinal tract of mice less efficiently than their parental strain, at long term. Comparative analysis of faecal 16S sequences indicated that T6SS-1 impaired the microbiota richness and its resilience capacity. Oscillospiraceae family members could be specific competitors for the long-term gut establishment of K. pneumoniae .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-21396-w