OsPP2C09 Is a Bifunctional Regulator in Both ABA-Dependent and Independent Abiotic Stress Signaling Pathways

Clade A Type 2C protein phosphatases (PP2CAs) negatively regulate abscisic acid (ABA) signaling and have diverse functions in plant development and in response to various stresses. In this study, we showed that overexpression of the rice ABA receptor OsPYL/RCAR3 reduces the growth retardation observ...

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Published in:International journal of molecular sciences 2021-01, Vol.22 (1), p.393
Main Authors: Min, Myung Ki, Kim, Rigyeong, Hong, Woo-Jong, Jung, Ki-Hong, Lee, Jong-Yeol, Kim, Beom-Gi
Format: Article
Language:English
Subjects:
aba-dependent/independent pathway
Abiotic stress
Abscisic acid
Abscisic Acid - metabolism
Deoxyribonucleic acid
DNA
dreb regulation
Drought
Droughts
Gene expression
Gene Expression Regulation, Plant
Growth rate
Kinases
Oryza - enzymology
Oryza - genetics
Oryza - physiology
Osmosis
Osmotic Pressure
Osmotic stress
Phosphorylation
Plant Proteins - metabolism
Plant Proteins - physiology
Polyethylene glycol
PP2CAS bifunction
Promoters
Protein Phosphatase 2C - metabolism
Protein Phosphatase 2C - physiology
Proteins
Protoplasts
Regulatory sequences
Rice
Seeds
Signal Transduction
Stress, Physiological
Transcription factors
Transcriptomes
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Summary:Clade A Type 2C protein phosphatases (PP2CAs) negatively regulate abscisic acid (ABA) signaling and have diverse functions in plant development and in response to various stresses. In this study, we showed that overexpression of the rice ABA receptor OsPYL/RCAR3 reduces the growth retardation observed in plants exposed to osmotic stress. By contrast, overexpression of the OsPYL/RCAR3-interacting protein OsPP2C09 rendered plant growth more sensitive to osmotic stress. We tested whether OsPP2CAs activate an ABA-independent signaling cascade by transfecting rice protoplasts with luciferase reporters containing the drought-responsive element (DRE) or ABA-responsive element (ABRE). We observed that OsPP2CAs activated gene expression via the cis-acting drought-responsive element. In agreement with this observation, transcriptome analysis of plants overexpressing OsPP2C09 indicated that OsPP2C09 induces the expression of genes whose promoters contain DREs. Further analysis showed that OsPP2C09 interacts with DRE-binding (DREB) transcription factors and activates reporters containing DRE. We conclude that, through activating DRE-containing promoters, OsPP2C09 positively regulates the drought response regulon and activates an ABA-independent signaling pathway.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22010393