Magnetic Bead Handling Using a Paper-Based Device for Quantitative Point-of-Care Testing

Microfluidic paper-based analytical devices (μPADs) have been extensively proposed as ideal tools for point-of-care (POC) testing with minimal user training and technical requirements. However, most μPADs use dried bioreagents, which complicate production, reduce device reproducibility and stability...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-08, Vol.12 (9), p.680
Main Authors: Arias-Alpízar, Kevin, Sánchez-Cano, Ana, Prat-Trunas, Judit, Sulleiro, Elena, Bosch-Nicolau, Pau, Salvador, Fernando, Oliveira, Inés, Molina, Israel, Sánchez-Montalvá, Adrián, Baldrich, Eva
Format: Article
Language:English
Subjects:
Antibodies
Automation
Beads
Biomarkers
Colorimetry
Controlled conditions
Dehydrogenases
Design and construction
Diagnosis
Diagnostic equipment (Medical)
Engineering research
Enzyme-linked immunosorbent assay
Granular materials
Image analysis
Image processing
immuno-modified magnetic beads
Immunoassay
Immunoassays
L-Lactate dehydrogenase
Lactate dehydrogenase
Lactate Dehydrogenases
Lactic acid
low-cost assay automation
Magnetic Phenomena
Magnetic properties
Malaria
malaria quantitative diagnosis
Measurement
Microfluidics
Microscopy
paper-based diagnostic device
Parasites
Plasmodium falciparum
Point-of-Care Testing
Reagents
Reproducibility of Results
Room temperature
Signal generation
smartphone colorimetric detection
Substrates
Temperature requirements
Vector-borne diseases
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Description
Summary:Microfluidic paper-based analytical devices (μPADs) have been extensively proposed as ideal tools for point-of-care (POC) testing with minimal user training and technical requirements. However, most μPADs use dried bioreagents, which complicate production, reduce device reproducibility and stability, and require transport and storage under temperature and humidity-controlled conditions. In this work, we propose a μPAD produced using an affordable craft-cutter and stored at room temperature, which is used to partially automate a single-step colorimetric magneto-immunoassay. As a proof-of-concept, the μPAD has been applied to the quantitative detection of Plasmodium falciparum lactate dehydrogenase (Pf-LDH), a biomarker of malaria infection. In this system, detection is based on a single-step magneto-immunoassay that consists of a single 5-min incubation of the lysed blood sample with immuno-modified magnetic beads (MB), detection antibody, and an enzymatic signal amplifier (Poly-HRP). This mixture is then transferred to a single-piece paper device where, after on-chip MB magnetic concentration and washing, signal generation is achieved by adding a chromogenic enzyme substrate. The colorimetric readout is achieved by the naked eye or using a smartphone camera and free software for image analysis. This μPAD afforded quantitative Pf-LDH detection in
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12090680