The Mitochondrial Genome of the Sea Anemone Stichodactyla haddoni Reveals Catalytic Introns, Insertion-Like Element, and Unexpected Phylogeny

A hallmark of sea anemone mitochondrial genomes (mitogenomes) is the presence of complex catalytic group I introns. Here, we report the complete mitogenome and corresponding transcriptome of the carpet sea anemone (family Stichodactylidae). The mitogenome is vertebrate-like in size, organization, an...

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Bibliographic Details
Published in:Life (Basel, Switzerland) Switzerland), 2021-04, Vol.11 (5), p.402
Main Authors: Johansen, Steinar Daae, Chi, Sylvia I, Dubin, Arseny, Jørgensen, Tor Erik
Format: Article
Language:English
Subjects:
Actiniaria
Cytochrome
Cytochrome-c oxidase
Cytochromes
Endonuclease
Gene expression
Genes
Genomes
Genomics
group I intron
Homing endonuclease
Insertion
Introns
Matematikk og Naturvitenskap: 400
Mathematics and natural science: 400
Mitochondria
Mitochondrial DNA
mitogenome
mtDNA
NADH
NADH dehydrogenase
Nicotinamide adenine dinucleotide
Nucleotides
Phylogenetics
Phylogeny
rearrangement
Ribonucleic acid
RNA
Stichodactyla haddoni
Taxonomy
Transcriptomes
VDP
Vertebrates
Zoologiske og botaniske fag: 480
Zoology and botany: 480
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Summary:A hallmark of sea anemone mitochondrial genomes (mitogenomes) is the presence of complex catalytic group I introns. Here, we report the complete mitogenome and corresponding transcriptome of the carpet sea anemone (family Stichodactylidae). The mitogenome is vertebrate-like in size, organization, and gene content. Two mitochondrial genes encoding NADH dehydrogenase subunit 5 (ND5) and cytochrome c oxidase subunit I (COI) are interrupted with complex group I introns, and one of the introns (ND5-717) harbors two conventional mitochondrial genes (ND1 and ND3) within its sequence. All the mitochondrial genes, including the group I introns, are expressed at the RNA level. Nonconventional and optional mitochondrial genes are present in the mitogenome of . One of these gene codes for a COI-884 intron homing endonuclease and is organized in-frame with the upstream COI exon. The insertion-like is expressed as RNA and translocated in the mitogenome as compared with other sea anemones. Phylogenetic analyses based on complete nucleotide and derived protein sequences indicate that is embedded within the family Actiniidae, a finding that challenges current taxonomy.
ISSN:2075-1729
2075-1729
DOI:10.3390/life11050402