Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and its Mycotoxin-Alleviating Effect on Pig Production Performance

• Published in: Toxins 2019-09, Vol.11 (10), p.552
• Main Authors: Liu, Chaoqi, Chang, Juan, Wang, Ping, Yin, Qingqiang, Huang, Weiwei, Dang, Xiaowei, Lu, Fushan, Gao, Tianzeng
• Format: Article
• Language: English
• Subjects: Animals; Aspergillus oryzae; Bacillus subtilis; Biodegradation; Candida utilis; cell-free extracts of Aspergillus oryzae; Cereals; Digestibility; Estrogen receptors; Estrogens; Feeds; Hogs; Immunoglobulins; Intestinal microflora; Microbiota; mRNA; Mycotoxins; Ovaries; pig production performance; Probiotics; Sp1 protein; Swine production; Uterus; Zearalenone
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins11100552

In order to remove zearalenone (ZEA) detriment- , and cell-free extracts from were used to degrade ZEA in this study. The orthogonal experiment in vitro showed that the ZEA degradation rate was 92.27% ( < 0.05) under the conditions that , SP1, and SP2 were mixed together at 0.5%, 1.0%, and 1.0%. When cell-free extracts from were combined with the above probiotics at a ratio of 2:1 to make mycotoxin-biodegradation preparation (MBP), the ZEA degradation rate reached 95.15% ( < 0.05). In order to further investigate the MBP effect on relieving the negative impact of ZEA for pig production performance, 120 young pigs were randomly divided into 5 groups, with 3 replicates in each group and 8 pigs for each replicate. Group A was given the basal diet with 86.19 μg/kg ZEA; group B contained 300 μg/kg ZEA without MBP addition; and groups C, D, and E contained 300 μg/kg ZEA added with 0.05%, 0.10%, and 0.15% MBP, respectively. The results showed that MBP addition was able to keep gut microbiota stable. ZEA concentrations in jejunal contents in groups A and D were 89.47% and 80.07% lower than that in group B ( < 0.05), indicating that MBP was effective in ZEA biodegradation. In addition, MBP had no significant effect on pig growth, nutrient digestibility, and the relative mRNA abundance of estrogen receptor alpha ( ) genes in ovaries and the uterus ( > 0.05).