Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica

Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has...

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Bibliographic Details
Published in:Scientific reports 2017-07, Vol.7 (1), p.6178-8, Article 6178
Main Authors: Abe, Yoshifumi, Van Nguyen, Khieu, Tsurugizawa, Tomokazu, Ciobanu, Luisa, Le Bihan, Denis
Format: Article
Language:English
Subjects:
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Animals
Aplysia - chemistry
Buccal ganglia
Cell activation
Cell size
Diffusion
Diffusion coefficient
Diffusion Magnetic Resonance Imaging
Dopamine
Functional magnetic resonance imaging
Humanities and Social Sciences
Intracellular
Light microscopy
Microscopy
multidisciplinary
Neuroimaging
Neuroimaging - methods
Neurons - cytology
Oxygen - blood
Oxygenation
Perfusion
Science
Science (multidisciplinary)
Water - chemistry
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Summary:Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has been hypothesized that the water apparent diffusion coefficient (ADC) decrease observed upon neural activation results from swelling of neurons or neuron parts. To elucidate the origin of the DfMRI response at cellular level we performed diffusion MR microscopy at 17.2 T in Aplysia californica buccal ganglia and compared the water ADCs at cellular and ganglia levels before and after neuronal activation induced by perfusion with a solution containing dopamine. Neural cell swelling, evidenced from optical microscopy imaging, resulted in an intracellular ADC increase and an ADC decrease at ganglia level. Furthermore, the intracellular ADC increase was found to have a significant positive correlation with the increase in cell size. Our results strongly support the hypothesis that the ADC decrease observed with DfMRI upon neuronal activation at tissue level reflects activation-induced neural cell swelling.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-05586-5