Isolation and Identification of Anthocyanin Component in the Fruits of Acanthopanax Sessiliflorus (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-04, Vol.25 (8), p.1781
• Main Authors: Chen, Liang, Xin, Xiulan, Feng, Hui, Li, Shuangshi, Cao, Qiguang, Wang, Xinying, Vriesekoop, Frank
• Format: Article
• Language: English
• Subjects: Acanthopanax; Acanthopanax sessiliflorus (Rupr. & Acids; Analgesics; anthocyanin; Anthocyanins; Anthocyanins - analysis; Anthocyanins - chemistry; Antidiabetics; Antihypertensives; antioxidant activity; Antioxidants; Antioxidants - analysis; Biphenyl Compounds - chemistry; Chromatography; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Diabetes mellitus; Eleutherococcus - chemistry; Fruit - chemistry; Fruits; Galactosides; Galactosides - analysis; Galactosides - chemistry; High performance liquid chromatography; high-speed counter-current chromatography (HSCCC); Inflammation; Ionization; Liquid chromatography; Magnetic Resonance Spectroscopy; Mass spectrometry; Mass spectroscopy; Maxim.) Seem; NMR; Nuclear magnetic resonance; Picrates - chemistry; Plant Extracts - analysis; Plant Extracts - chemistry; Purification; Scavenging; Sugar
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25081781

(Rupr. & Maxim.) Seem. ( ) is one of the most abundant species of genus . The fruits of are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of . The anthocyanin content in the fresh fruits of was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), H- and C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.