Salmonirosea aquatica gen. nov., sp. nov., a Novel Genus within the Family Spirosomaceae , Was Isolated from Brackish Water in the Republic of Korea

• Published in: Microorganisms (Basel) 2024-08, Vol.12 (8), p.1671
• Main Authors: Baek, Kiwoon, Jang, Sumin, Goh, Jaeduk, Choi, Ahyoung
• Format: Article
• Language: English
• Subjects: Biodegradation; Biodiversity; Brackish water; chemotaxonomy; Cytophagales; Ecosystems; family; Fatty acids; Fresh water; Genes; Genomes; genomic analysis; Genomics; lipid composition; Lipids; Menaquinones; Microscopy; New genera; new genus; New species; novel genus; novel species; phenotype; Phosphatidylethanolamine; phosphatidylethanolamines; Phylogenetics; Phylogeny; Pollutants; River ecology; rivers; rRNA 16S; Salmonirosea; Salmonirosea aquatica; Sodium chloride; Software; South Korea; species; Strain analysis; taxonomy; Temperature; Urbanization; Water analysis; Water sampling
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12081671

A Gram-stain-negative, obligately aerobic, non-motile, rod-shaped bacterial strain designated SJW1-29 was isolated from brackish water samples collected from the Seomjin River, Republic of Korea. The purpose of this study was to characterize strain SJW1-29 and determine its taxonomic position as a potential new genus within the family . The strain grew within the range of 10-30 °C (optimum, 25 °C), pH 5.0-10.0 (optimum, 7.0), and 1-4% NaCl (optimum, 3%). Phylogenetic analysis based on the 16S rRNA gene revealed that strain SJW1-29 belongs to the family and is closely related to Shu-9-SY12-35C (91.3% similarity), R491 (90.6%), and DMA-K-7a (90.0%), while the similarities to strains within the order were lower than 90.0%. The genome is 7.1 Mbp with a G+C content of 50.7 mol%. The use of genome-relatedness indices confirmed that this strain belongs to a new genus. The major polar lipid profile consisted of phosphatidylethanolamine, and MK-7 was the predominant menaquinone. The predominant fatty acids were summed feature 3 (C 7 and/or C 6 ), iso-C , iso-C 3-OH, and C , representing more than 80% of the total fatty acids. The phenotypic, chemotaxonomic, genetic, and phylogenetic properties suggest that strain SJW1-29 represents a novel species within a new genus in the family , for which the name gen. nov., sp. nov., is proposed. The type strain of is SJW1-29 (=KCTC 72493 = NBRC 114061 = FBCC-B16924 ).