Comprehensive analysis of silk proteins and gland compartments in Limnephilus lunatus, a case-making trichopteran

Caddisfly larvae produce silk containing heavy and light fibroins, similar to the silk of Lepidoptera, for the construction of underwater structures. We analyzed the silk of Limnephilus lunatus belonging to the case-forming suborder Integripalpia. We analyzed the transcriptome, mapped the transcript...

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Bibliographic Details
Published in:BMC genomics 2024-05, Vol.25 (1), p.472-472, Article 472
Main Authors: Rouhova, Lenka, Zurovcova, Martina, Hradilova, Miluse, Sery, Michal, Sehadova, Hana, Zurovec, Michal
Format: Article
Language:English
Subjects:
Adhesives
Analysis
Animals
Aquatic insects
Butterflies & moths
Caddis-flies
Candidates
Control
Enzymes
Fibroin
Fibroins - chemistry
Fibroins - genetics
Fibroins - metabolism
Gene duplication
Gene Expression Profiling
Genetic aspects
Genomes
Genomics
Hydrophobicity
Identification and classification
Insect Proteins - genetics
Insect Proteins - metabolism
Insecta - genetics
Insecta - metabolism
Larvae
Limnephilus flavicornis
Limnephilus lunatus
Localization
Peptides
Plectrocnemia conspersa
Production processes
Proteins
Proteome
Proteomes
Proteomics
Proteomics - methods
Sericin
Silk
Silk - chemistry
Silk - metabolism
Silk gland
Transcriptome
Transcriptomes
Underwater construction
Underwater structures
Webs
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Description
Summary:Caddisfly larvae produce silk containing heavy and light fibroins, similar to the silk of Lepidoptera, for the construction of underwater structures. We analyzed the silk of Limnephilus lunatus belonging to the case-forming suborder Integripalpia. We analyzed the transcriptome, mapped the transcripts to a reference genome and identified over 80 proteins using proteomic methods, and checked the specificity of their expression. For comparison, we also analyzed the transcriptome and silk proteome of Limnephilus flavicornis. Our results show that fibroins and adhesives are produced together in the middle and posterior parts of the silk glands, while the anterior part produces enzymes and an unknown protein AT24. The number of silk proteins of L. lunatus far exceeds that of the web-spinning Plectrocnemia conspersa, a previously described species from the suborder Annulipalpia. Our results support the idea of increasing the structural complexity of silk in rigid case builders compared to trap web builders.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-024-10381-4