The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression

• Published in: Journal of obstetrics and gynaecology 2024-12, Vol.44 (1), p.2360547
• Main Authors: Cao, Ming-Gang, Wang, Yan, Yang, Zhi-Min, Wang, Yang, Wang, Mei-Qing, Zhuo, Shuai, Yang, Yan, Liu, Chun-Sheng
• Format: Article
• Language: English
• Subjects: Adult; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - mortality; Breast Neoplasms - pathology; Cell Line, Tumor; Cell Proliferation - genetics; cyclin A2; Cyclin A2 - genetics; Cyclin A2 - metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; MCF-7 Cells; Medical prognosis; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; migration; miR-381; Prognosis; proliferation
• ISSN: 0144-3615; 1364-6893; 1364-6893
• DOI: 10.1080/01443615.2024.2360547

MiR-381 can regulate the expression of cyclin A2 (CCNA2) to inhibit the proliferation and migration of bladder cancer cells, but whether miR-381 has the same function in breast cancer is not well know. The over express or silence miR-381 expressing cell lines were constructed by lentivirus infection to reveal the biological functions of miR-381 . The expression of miR-381 and CCNA2 in 162 breast cancer patients were detected to further reveal their impact and predictive value on progression-free survival (PFS) and overall survival (OS). After transfection of MDA-MB-231 and MCF-7 cells with miR-381 mimics, the expression of miR-381 was effectively up-regulated and CCNA2 was effectively down-regulated, while the opposite results were observed in tumour cell which transfected with miR-381 inhibitors. After transfection of cell lines with miR-381 mimics, tumour cell activity was significantly reduced, while the opposite results were observed in tumour cell which transfected with miR-381 inhibitors. The area under curves (AUCs) of miRNA-381 and CCNA2 for predicting PFS and OS were 0.711, 0.695, 0.694 and 0.675 respectively. Cox regression analysis showed that miRNA-381 ≥ 1.65 2 and CCNA ≥ 2.95 2 were the influence factors of PFS and OS, the hazard ratio (HR) values were 0.553, 2.075, 0.462 and 2.089, respectively. miR-381 inhibitors breast cancer cells proliferation and migration by down-regulating the expression of CCNA2, both of them can predict the prognosis of breast cancer.