Post-Consumer Poly(ethylene terephthalate) (PET) Depolymerization by Yarrowia lipolytica : A Comparison between Hydrolysis Using Cell-Free Enzymatic Extracts and Microbial Submerged Cultivation

Several microorganisms have been reported as capable of acting on poly(ethylene terephthalate) (PET) to some extent, such as , which is a yeast known to produce various hydrolases of industrial interest. The present work aims to evaluate PET depolymerization by using two different strategies. In the...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2022-11, Vol.27 (21), p.7502
Main Authors: Sales, Julio Cesar Soares, de Castro, Aline Machado, Ribeiro, Bernardo Dias, Coelho, Maria Alice Zarur
Format: Article
Language:English
Subjects:
Biocatalysis
Biocatalysts
Biodegradation
Bioreactors
Cell culture
Cell Extracts
Culture media
Depolymerization
Environmental impact
Enzymes
Ethylene
Ethylenes
Fermentation
Hydrolysis
Landfill
lipase
Metabolism
Microorganisms
plastics
Polyethylene terephthalate
Polyethylene Terephthalates
Solid state fermentation
Terephthalic acid
Yarrowia
Yarrowia lipolytica
Yeast
Yeasts
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Summary:Several microorganisms have been reported as capable of acting on poly(ethylene terephthalate) (PET) to some extent, such as , which is a yeast known to produce various hydrolases of industrial interest. The present work aims to evaluate PET depolymerization by using two different strategies. In the first one, biocatalysts were produced during solid-state fermentation (SSF-YL), extracted and subsequently used for the hydrolysis of PET and bis(2-hydroxyethyl terephthalate) (BHET), a key intermediate in PET hydrolysis. Biocatalysts were able to act on BHET, yielding terephthalic acid (TPA) (131.31 µmol L ), and on PET, leading to a TPA concentration of 42.80 µmol L after 168 h. In the second strategy, PET depolymerization was evaluated during submerged cultivations of using four different culture media, and the use of YT medium (( / ) yeast extract 1%, tryptone 2%) yielded the highest TPA concentration after 96 h (65.40 µmol L ). A final TPA concentration of 94.3 µmol L was obtained on a scale-up in benchtop bioreactors using YT medium. The conversion obtained in bioreactors was 121% higher than in systems with SSF-YL. The results of the present work suggest a relevant role of cells in the depolymerization process.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27217502