Phosphite Application Alleviates Pythophthora infestans by Modulation of Photosynthetic and Physio-Biochemical Metabolites in Potato Leaves

• Published in: Pathogens (Basel) 2020-02, Vol.9 (3), p.170
• Main Authors: Mohammadi, Mohammad Aqa, Han, Xiaoyun, Zhang, Zhizhong, Xi, Yupei, Boorboori, Mohammadreza, Wang-Pruski, Gefu
• Format: Article
• Language: English
• Subjects: antioxidant compounds; chlorophyll; late blight; oxidative enzymes; phytophtora infestans; potassium phosphite; potato
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens9030170

Potato late blight ( ) is among the most severely damaging diseases of potato ( L.) worldwide, causing serious damages in potato leaves and tubers. In the present study, the effects of potassium phosphite (KPhi) applications on photosynthetic parameters, enzymatic and non-enzymatic antioxidant properties, hydrogen peroxide (H O ) and malondialdehyde (MDA), total protein and total carbohydrate of potato leaves challenged with pathogen were investigated. Potato leaves were sprayed five times with KPhi (0.5%) during the growing season prior to inoculation with . The potato leaves were artificially infected by the LC06-44 pathogen isolate. The leaves were sampled at 0, 24, 48, 72 and 96 h after the infection for evaluations. infection reduced chlorophyll (Chl) pigments contents, chlorophyll fluorescence, carotenoid (Car) and anthocyanin contents and increased the accumulation of H O and MDA. Meanwhile, our result showed that KPhi treatment alleviated adverse effect of late blight in potato leaves. KPhi application also increased plant tolerance to the pathogen with improved photosynthetic parameters Chl , total Chl, Car, and anthocyanin compare to controls. Moreover, the increased oxidative enzymes activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APx), and non-enzymatic substances such as phenolics, flavonoids and proline were found in KPhi treated plants, compared to untreated plants after inoculation. In addition, KPhi application followed by infection also decreased the content of H O and MDA, but increased the total protein and total carbohydrate contents in potato leaves. The consequence of current research indicated that KPhi played a vital role in pathogen tolerance, protecting the functions of photosynthetic apparatus by improved oxidative levels and physio-biochemical compounds in potato leaves.