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Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages

•Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans. Oxidized LDL (ox-LDL) and its interaction with lectin type ox-L...

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Bibliographic Details
Published in:Journal of functional foods 2016-06, Vol.24, p.97-111
Main Authors: Alves, Natália E.G., Vasconcelos, Christiane M., Bassinello, Priscila Z., de Mejia, Elvira G., Martino, Hércia S.D.
Format: Article
Language:English
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Summary:•Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans. Oxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in human THP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin–pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-α (TNF-α) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1.
ISSN:1756-4646
2214-9414
DOI:10.1016/j.jff.2016.03.027