Loading…
Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages
•Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans. Oxidized LDL (ox-LDL) and its interaction with lectin type ox-L...
Saved in:
Published in: | Journal of functional foods 2016-06, Vol.24, p.97-111 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53 |
---|---|
cites | cdi_FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53 |
container_end_page | 111 |
container_issue | |
container_start_page | 97 |
container_title | Journal of functional foods |
container_volume | 24 |
creator | Alves, Natália E.G. Vasconcelos, Christiane M. Bassinello, Priscila Z. de Mejia, Elvira G. Martino, Hércia S.D. |
description | •Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans.
Oxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in human THP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin–pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-α (TNF-α) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1. |
doi_str_mv | 10.1016/j.jff.2016.03.027 |
format | article |
fullrecord | <record><control><sourceid>elsevier_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_9b658dc4795140ee8d551882c7c6740e</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1756464616300482</els_id><doaj_id>oai_doaj_org_article_9b658dc4795140ee8d551882c7c6740e</doaj_id><sourcerecordid>S1756464616300482</sourcerecordid><originalsourceid>FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53</originalsourceid><addsrcrecordid>eNp9UcFO3DAUtKoidQt8ADf_QFLbcexEPVULBaSV6IGeLeflmXW6xCs7RS1_wF_zlkU99vJsjd6MZt4wdiFFLYU0X6Z6CqFW9K1FUwtlP7CVUlJXvZb6I1tJ25pKG20-sc-lTEIYIxuxYi-X8QHLgiPf57RgnHksaecX5CGnRxpYttzPIy9LyrS19jkm8HxAPxdOyG8g9NHnX5gLT4H7ZYs5FdgdZiycFNOfOMZnWttcbqo4Hyn3Nz8qSUTIab_15OGMnQS_K3j-_p6yn9-v7tc31ebu-nb9bVOBst1SSQvYewsAQguphk4GsDqM7ahx0GowqEA2XdtKDEZDoF2rQHe9DK1poG1O2e1Rd0x-cvscyfxfl3x0b0DKD87nJVIA1w-m7UbQtm-lFojdSLJdp8CCsQSQljxqUYpSMoZ_elK4Qy9uctSLO_TiROOoF-J8PXKQQj5FzK5AxJluEjPCQi7if9ivC7CXRw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages</title><source>Elsevier</source><creator>Alves, Natália E.G. ; Vasconcelos, Christiane M. ; Bassinello, Priscila Z. ; de Mejia, Elvira G. ; Martino, Hércia S.D.</creator><creatorcontrib>Alves, Natália E.G. ; Vasconcelos, Christiane M. ; Bassinello, Priscila Z. ; de Mejia, Elvira G. ; Martino, Hércia S.D.</creatorcontrib><description>•Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans.
Oxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in human THP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin–pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-α (TNF-α) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1.</description><identifier>ISSN: 1756-4646</identifier><identifier>EISSN: 2214-9414</identifier><identifier>DOI: 10.1016/j.jff.2016.03.027</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Bioactive peptides ; Carioca beans ; Common bean ; Lectin type ox-LDL receptor 1 ; Oxidized LDL</subject><ispartof>Journal of functional foods, 2016-06, Vol.24, p.97-111</ispartof><rights>2016 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53</citedby><cites>FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Alves, Natália E.G.</creatorcontrib><creatorcontrib>Vasconcelos, Christiane M.</creatorcontrib><creatorcontrib>Bassinello, Priscila Z.</creatorcontrib><creatorcontrib>de Mejia, Elvira G.</creatorcontrib><creatorcontrib>Martino, Hércia S.D.</creatorcontrib><title>Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages</title><title>Journal of functional foods</title><description>•Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans.
Oxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in human THP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin–pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-α (TNF-α) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1.</description><subject>Bioactive peptides</subject><subject>Carioca beans</subject><subject>Common bean</subject><subject>Lectin type ox-LDL receptor 1</subject><subject>Oxidized LDL</subject><issn>1756-4646</issn><issn>2214-9414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9UcFO3DAUtKoidQt8ADf_QFLbcexEPVULBaSV6IGeLeflmXW6xCs7RS1_wF_zlkU99vJsjd6MZt4wdiFFLYU0X6Z6CqFW9K1FUwtlP7CVUlJXvZb6I1tJ25pKG20-sc-lTEIYIxuxYi-X8QHLgiPf57RgnHksaecX5CGnRxpYttzPIy9LyrS19jkm8HxAPxdOyG8g9NHnX5gLT4H7ZYs5FdgdZiycFNOfOMZnWttcbqo4Hyn3Nz8qSUTIab_15OGMnQS_K3j-_p6yn9-v7tc31ebu-nb9bVOBst1SSQvYewsAQguphk4GsDqM7ahx0GowqEA2XdtKDEZDoF2rQHe9DK1poG1O2e1Rd0x-cvscyfxfl3x0b0DKD87nJVIA1w-m7UbQtm-lFojdSLJdp8CCsQSQljxqUYpSMoZ_elK4Qy9uctSLO_TiROOoF-J8PXKQQj5FzK5AxJluEjPCQi7if9ivC7CXRw</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Alves, Natália E.G.</creator><creator>Vasconcelos, Christiane M.</creator><creator>Bassinello, Priscila Z.</creator><creator>de Mejia, Elvira G.</creator><creator>Martino, Hércia S.D.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>201606</creationdate><title>Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages</title><author>Alves, Natália E.G. ; Vasconcelos, Christiane M. ; Bassinello, Priscila Z. ; de Mejia, Elvira G. ; Martino, Hércia S.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bioactive peptides</topic><topic>Carioca beans</topic><topic>Common bean</topic><topic>Lectin type ox-LDL receptor 1</topic><topic>Oxidized LDL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alves, Natália E.G.</creatorcontrib><creatorcontrib>Vasconcelos, Christiane M.</creatorcontrib><creatorcontrib>Bassinello, Priscila Z.</creatorcontrib><creatorcontrib>de Mejia, Elvira G.</creatorcontrib><creatorcontrib>Martino, Hércia S.D.</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of functional foods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alves, Natália E.G.</au><au>Vasconcelos, Christiane M.</au><au>Bassinello, Priscila Z.</au><au>de Mejia, Elvira G.</au><au>Martino, Hércia S.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages</atitle><jtitle>Journal of functional foods</jtitle><date>2016-06</date><risdate>2016</risdate><volume>24</volume><spage>97</spage><epage>111</epage><pages>97-111</pages><issn>1756-4646</issn><eissn>2214-9414</eissn><abstract>•Peptides from bean digested protein isolates reduced atherosclerotic markers.•Bean peptides inhibit markers of LOX-1 signalling pathway in THP-1 macrophages.•Short storage did not impair anti-atherosclerotic potential of Carioca beans.
Oxidized LDL (ox-LDL) and its interaction with lectin type ox-LDL receptor (LOX-1) determine atherosclerosis progression. The potential of peptides from digested protein isolate (DPI) derived from fresh and stored Carioca bean (Phaseolus vulgaris L.) to prevent ox-LDL induced inflammation in human THP-1 macrophage-like cells was examined here. BRS Pontal (PO) and BRSMG Madreperola (MP) fresh and stored for 6 months were cooked, and protein isolates were digested by pepsin–pancreatin. Peptides from stored Carioca beans decreased prostaglandin E-2 (PGE-2) (from 17.7 to 47%) and tumour necrosis factor-α (TNF-α) (12.3 to 82%). Stored MP (0.01 mg/mL) inhibited reactive oxygen species (ROS). LOX-1 and matrix metalloproteinase-9 (MMP-9) expression were reduced by peptides from all beans. Stored PO and MP digested protein isolates reduced expression of 10 cytokines related to atherosclerosis process. Peptides from Carioca beans presented anti-atherosclerotic properties comparable to simvastatin, which were related to inhibition of LOX-1, MMP-9 and ICAM-1.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.jff.2016.03.027</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1756-4646 |
ispartof | Journal of functional foods, 2016-06, Vol.24, p.97-111 |
issn | 1756-4646 2214-9414 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_9b658dc4795140ee8d551882c7c6740e |
source | Elsevier |
subjects | Bioactive peptides Carioca beans Common bean Lectin type ox-LDL receptor 1 Oxidized LDL |
title | Digested protein isolate from fresh and stored Carioca beans reduced markers of atherosclerosis in oxidized LDL-induced THP-1 macrophages |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T12%3A30%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-elsevier_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Digested%20protein%20isolate%20from%20fresh%20and%20stored%20Carioca%20beans%20reduced%20markers%20of%20atherosclerosis%20in%20oxidized%20LDL-induced%20THP-1%20macrophages&rft.jtitle=Journal%20of%20functional%20foods&rft.au=Alves,%20Nat%C3%A1lia%20E.G.&rft.date=2016-06&rft.volume=24&rft.spage=97&rft.epage=111&rft.pages=97-111&rft.issn=1756-4646&rft.eissn=2214-9414&rft_id=info:doi/10.1016/j.jff.2016.03.027&rft_dat=%3Celsevier_doaj_%3ES1756464616300482%3C/elsevier_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c278t-17ce9a7ccc04012b81fc74fd5d4eb42b6e2c138551ef64cf9a772c4891f563c53%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |