Determination of peracetylated sulfoglycolipids using the azure A method

Several sulfoglycolipids, including sulfogalactosylceramide, sulfolactosylceramide, monosulfogangliotriaosylceramide, monosulfogangliotetraosylceramide, bis-sulfogangliotriaosylceramide, bis-sulfogangliotetraosylceramide, seminolipid, and lysoseminolipid, were assayed by the azure A procedure (Kean,...

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Bibliographic Details
Published in:Journal of lipid research 1983-10, Vol.24 (10), p.1368-1375
Main Authors: Tadano-Aritomi, K, Ishizuka, I
Format: Article
Language:English
Subjects:
Acetylation
Animals
Azure Stains
Chromatography, Thin Layer - methods
Glycolipids - analysis
Humans
Kidney - analysis
Rats
Spectrophotometry
Staining and Labeling
Structure-Activity Relationship
Sulfoglycosphingolipids - analysis
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Summary:Several sulfoglycolipids, including sulfogalactosylceramide, sulfolactosylceramide, monosulfogangliotriaosylceramide, monosulfogangliotetraosylceramide, bis-sulfogangliotriaosylceramide, bis-sulfogangliotetraosylceramide, seminolipid, and lysoseminolipid, were assayed by the azure A procedure (Kean, E.L. 1968. J. Lipid Res. 9: 319-327). The color yields were different significantly among each sulfoglycolipid. The accurate determination of monosulfoglycolipids having longer carbohydrate chains than sulfolactosylceramide as well as bis-sulfoglycolipids could not be achieved because of the low color yields, turbidity, and/or formation of an additional chromogen. However, the quantitative determination of these complex sulfoglycolipids could be achieved using the azure A procedure when these compounds were assayed after peracetylation. Using the modified Kean procedure, the behavior of these sulfoglycolipids in the chloroform-methanol-water partition system was compared.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)37887-1