Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries

Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica a...

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Bibliographic Details
Published in:Veterinary Medicine International 2010, Vol.2010 (2010), p.310-313
Main Authors: Cremonesi, Fausto, Lange-Consiglio, A., Anderson, K.
Format: Article
Language:English
Subjects:
Catheters
Fertilization in vitro
Oocytes
Ovaries
Physiological aspects
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Summary:Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica albuginea removal. This hollow hypodermic needle, usually employed for inserting epidural catheters, is designed with a slightly curved tip, shaped similar to a small curette. In unpeeled ovaries, the recovery rates of Tuohy needle group was higher (P
ISSN:2090-8113
2042-0048
2042-0048
DOI:10.4061/2010/102591