Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes

• Published in: Nature communications 2024-09, Vol.15 (1), p.7627-17, Article 7627
• Main Authors: Wang, Hong-Yan, Chen, Jian-Yang, Li, Yanan, Zhang, Xianghui, Liu, Xiang, Lu, Yifang, He, Hang, Li, Yubang, Chen, Hongxi, Liu, Qun, Huang, Yingyi, Jia, Zhao, Li, Shuo, Zhang, Yangqing, Han, Shenglei, Jiang, Shuhong, Yang, Mingming, Zhang, Yingying, Zhou, Li, Tan, Fujian, Ji, Qianyue, Meng, Liang, Wang, Rui, Liu, Yuyan, Liu, Kaiqiang, Wang, Qian, Seim, Inge, Zou, Jun, Fan, Guangyi, Liu, Shanshan, Shao, Changwei
• Format: Article
• Language: English
• Subjects: 13/31; 14/32; 45/90; 49/91; 631/114/2114; 631/181/2806; 631/250/2152; 631/250/248; Adaptive immunity; Adaptive systems; Animals; B-Lymphocytes - cytology; Bamboo; Biological Evolution; Conserved sequence; Evolutionary conservation; Fishes - classification; Fishes - genetics; Fishes - immunology; Gene sequencing; Humanities and Social Sciences; Immune system; Lymphocytes; Lymphocytes - cytology; Lymphocytes T; multidisciplinary; Ontogeny; Pattern recognition; Pattern recognition receptors; Phagocytes; Phagocytosis; Receptor mechanisms; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Sharks; Sharks - immunology; Single-Cell Gene Expression Analysis; Sole; Species; Species comparisons; T-Lymphocyte Subsets - cytology; T-Lymphocytes - cytology; Vertebrates; Zebrafish; Zebrafish - immunology
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-51761-4

Elucidating cellular architecture and cell-type evolution across species is central to understanding immune system function and susceptibility to disease. Adaptive immunity is a shared trait of the common ancestor of cartilaginous and bony fishes. However, evolutionary features of lymphocytes in these two jawed vertebrates remain unclear. Here, we present a single-cell RNA sequencing atlas of immune cells from cartilaginous (white-spotted bamboo shark) and bony (zebrafish and Chinese tongue sole) fishes. Cross-species comparisons show that the same cell types across different species exhibit similar transcriptional profiles. In the bamboo shark, we identify a phagocytic B cell population expressing several pattern recognition receptors, as well as a T cell sub-cluster co-expressing both T and B cell markers. In contrast to a division by function in the bony fishes, we show close linkage and poor functional specialization among lymphocytes in the cartilaginous fish. Our cross-species single-cell comparison presents a resource for uncovering the origin and evolution of the gnathostome immune system. Both cartilaginous and bony fishes have adaptive immune cells, but the evolutionary features of these diverse cell populations remain understudied. Here the authors report single-cell RNA sequencing atlas of both species to define these features and to find reduced evolutionary diversification of immune cells in cartilaginous fishes.