First molecular evidence for Puumala hantavirus in Poland

Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European...

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Bibliographic Details
Published in:Viruses 2014-01, Vol.6 (1), p.340-353
Main Authors: Ali, Hanan Sheikh, Drewes, Stephan, Sadowska, Edyta T, Mikowska, Magdalena, Groschup, Martin H, Heckel, Gerald, Koteja, Pawel, Ulrich, Rainer G
Format: Article
Language:English
Subjects:
Animals
Antigens
Arvicolinae
bank vole
Brief Report
Clethrionomys (Myodes) glareolus
Cluster Analysis
Electronic mail systems
Fatalities
Hantavirus
Infections
Molecular Sequence Data
Phylogeny
Poland
Puumala virus
Puumala virus - classification
Puumala virus - genetics
Puumala virus - isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - genetics
Rodents
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Thoracic Cavity - virology
Viruses
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Summary:Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland.
ISSN:1999-4915
1999-4915
DOI:10.3390/v6010340