The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit Clostridium perfringens Growth

Clostridium perfringens is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a C. perfringens-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from chicken farm sewage. Both electron mi...

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Bibliographic Details
Published in:Viruses 2022-03, Vol.14 (4), p.676
Main Authors: Huang, Sisi, Tian, Yuan, Wang, Yongjuan, García, Pilar, Liu, Banhong, Lu, Rui, Wu, Liting, Bao, Hongduo, Pang, Maoda, Zhou, Yan, Wang, Ran, Zhang, Hui
Format: Article
Language:English
Subjects:
Animals
Antibiotics
antimicrobial agent
Antimicrobial agents
Bacteria
Bacterial infections
Bacteriophages
Bans
Chloramphenicol
Clostridium perfringens
Clostridium perfringens - genetics
Dogs
Electron microscopy
Enteritis
Feeds
Food contamination & poisoning
Foodborne diseases
Gangrene
Genome, Viral
Genomes
Homology
Host range
Host Specificity
Infections
Mortality
Nucleotide sequence
Open reading frames
Pathogens
phage
Phages
Phylogeny
Poultry
poultry industry
Proteins
Sequence analysis
Sewage
Siphoviridae - genetics
Temperature tolerance
Toxins
tRNA
Virulence
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Summary:Clostridium perfringens is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a C. perfringens-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from chicken farm sewage. Both electron microscopy and phylogenetic analysis suggested that phage BG3P is a novel phage belonging to Siphoviridae family. Phage BG3P exhibited a broad host range against different C. perfringens isolates (90.63% of strains were infected). Sequencing of the complete genome revealed a linear double-stranded DNA (43,528 bp) with 28.65% GC content. After sequence analysis, 73 open reading frames (orfs) were predicted, of which only 13 were annotated with known functions. No tRNA and virulence encoding genes were detected. It should be noted that the protein of orf 15 has 97.92% homology to C. perfringens-specific chloramphenicol resistance protein, which has not been reported for any C. perfringens phage. Phylogenetic analysis of the ssDNA binding protein demonstrated that this phage is closely related to C. perfringens phages phiSM101 and phi3626. In considering future use as an antimicrobial agent, some biological characteristics were observed, such as a good pH (3−11) stability and moderate temperature tolerance (
ISSN:1999-4915
1999-4915
DOI:10.3390/v14040676