Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications

Two-photon polymerization (TPP) is capable of fabricating 3D structures with dimensions from sub-µm to a few hundred µm. As a direct laser writing (DLW) process, fabrication time of 3D TPP structures scale with the third order, limiting its use in large volume fabrication. Here, we report on a scala...

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Bibliographic Details
Published in:Scientific reports 2020-05, Vol.10 (1), p.8740-8740, Article 8740
Main Authors: Maibohm, Christian, Silvestre, Oscar F., Borme, Jérôme, Sinou, Maina, Heggarty, Kevin, Nieder, Jana B.
Format: Article
Language:English
Subjects:
639/166/985
639/624
639/624/1075
639/624/1075/1080
Cell adhesion & migration
Cell migration
Cell proliferation
Cytology
Electromagnetism
Engineering Sciences
Fabrication
Hatching
Humanities and Social Sciences
Micro and nanotechnologies
Microelectronics
multidisciplinary
Optics
Photonic
Polymerization
Science
Science (multidisciplinary)
Signal and Image processing
Tissue engineering
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Summary:Two-photon polymerization (TPP) is capable of fabricating 3D structures with dimensions from sub-µm to a few hundred µm. As a direct laser writing (DLW) process, fabrication time of 3D TPP structures scale with the third order, limiting its use in large volume fabrication. Here, we report on a scalable fabrication method that cuts fabrication time to a fraction. A parallelized 9 multi-beamlets DLW process, created by a fixed diffraction optical element (DOE) and subsequent stitching are used to fabricate large periodic high aspect ratio 3D microstructured arrays with sub-micron features spanning several hundred of µm 2 . The wall structure in the array is designed with a minimum of traced lines and is created by a low numerical aperture (NA) microscope objective, leading to self-supporting lines omitting the need for line-hatching. The fabricated periodic arrays are applied in a cell – 3D microstructure interaction study using living HeLa cells. First indications of increased cell proliferation in the presence of 3D microstructures compared to planar surfaces are obtained. Furthermore, the cells adopt an elongated morphology when attached to the 3D microstructured surfaces. Both results constitute promising findings rendering the 3D microstructures a suited tool for cell interaction experiments, e.g. for cell migration, separation or even tissue engineering studies.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-64955-9