One-sample two-smear versus two-sample two-smear approach for the diagnosis of pulmonary tuberculosis

ABSTRACT BACKGROUND: To evaluate the efficacy of one-sputum sample two-smear approach for the diagnosis of pulmonary tuberculosis (PT). MATERIALS AND METHODS: Data from January 2012 to December 2015 were analyzed to find (1) number of smear positives (SP s ) by spot (S) sample with one and two smear...

Full description

Saved in:
Bibliographic Details
Published in:Journal of laboratory physicians 2018-04, Vol.10 (2), p.135-139
Main Author: Chandra, T. Jaya
Format: Article
Language:English
Subjects:
Analysis
Diagnosis
Health services
Light emitting diodes
Methods
Microscopy
Original
Original Article
Patients
Pulmonary tuberculosis
Risk factors
sample
staining
Stains & staining
Tuberculosis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT BACKGROUND: To evaluate the efficacy of one-sputum sample two-smear approach for the diagnosis of pulmonary tuberculosis (PT). MATERIALS AND METHODS: Data from January 2012 to December 2015 were analyzed to find (1) number of smear positives (SP s ) by spot (S) sample with one and two smears; (2) number of SP s by morning (M) sample with one and two smears; and (iii) number of SP s by two samples with two smears, that is, same-day (SS 2 ) and spot morning (SM) approaches. The Chi-square test was used to evaluate the statistical difference in SP cases. RESULTS: With one-sample two-smear approach, the smear positivity (SPT) was 87% and 87.5%, for S and M samples, respectively, for Ziehl–Neelsen (ZN) staining; whereas, SPT was 96% and 97%, respectively, for S and M samples, for fluorescent staining (FS) technique. With two-sample two-smear approach, for ZN staining, SPT was 89% each and for FS technique, SPT was 97% and 99%, respectively, for SS 2 and SM approaches. The difference was not statistically significant ( P > 0.05) between one- and two-sample approaches in the staining techniques. CONCLUSION: Significant number of SP cases are identified by S sample two-smear approach. Thus, the World Health Organization/Revised National Tuberculosis Control Programme can initiate S sample two-smear approach for the diagnosis of PT.
ISSN:0974-2727
0974-7826
DOI:10.4103/JLP.JLP_145_17